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Literature summary for 3.2.1.133 extracted from
- Association of novel domain in active site of archaic hyperthermophilic maltogenic amylase from Staphylothermus marinus (2012), J. Biol. Chem., 287, 7979-7989.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli MC1061 cells | Staphylothermus marinus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 2% (v/v) isopropyl alcohol, 0.1 M ADA, pH 6.5, and 0.1 M Li2SO4 | Staphylothermus marinus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus |  |
| 1.48 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 2.68 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylothermus marinus | A3DM60 | - |
- |
| Staphylothermus marinus DSM 3639 | A3DM60 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Staphylothermus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
| alpha-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? | |
| amylopectin + H2O | - |
Staphylothermus marinus | maltose + D-glucose | - |
? | |
| amylopectin + H2O | - |
Staphylothermus marinus DSM 3639 | maltose + D-glucose | - |
? | |
| amylose + H2O | - |
Staphylothermus marinus | maltose + D-glucose | - |
? | |
| amylose + H2O | - |
Staphylothermus marinus DSM 3639 | maltose + D-glucose | - |
? | |
| beta-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
| beta-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? | |
| gamma-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
| gamma-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltogenic amylase | - |
Staphylothermus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Staphylothermus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 109 | - |
109°C melting temperature with enzymatic activity under acidic conditions (pH 3.5-5.0) | Staphylothermus marinus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
amylose | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 0.8 | - |
amylopectin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 1.45 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 5.36 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 29.1 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.37 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 3.62 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
| 10.86 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
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