Protein Variants | Comment | Organism |
---|---|---|
G312A | the mutant has an optimal temperature of 45°C instead of the 40°C for the wild type enzyme | Bacillus sp. (in: Bacteria) |
G312A/K436R | the half-life time at 55°C increase from 15 to 25 min for the double mutant | Bacillus sp. (in: Bacteria) |
K436R | the mutant has an optimal temperature of 45°C instead of the 40°C for the wild type enzyme | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67500 | - |
x * 67500, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A7DWA8 | - |
- |
Bacillus sp. (in: Bacteria) US149 | A7DWA8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + H2O | highest catalytic efficiency | Bacillus sp. (in: Bacteria) | alpha-maltose + alpha-D-glucose | - |
? | |
beta-cyclodextrin + H2O | highest catalytic efficiency | Bacillus sp. (in: Bacteria) US149 | alpha-maltose + alpha-D-glucose | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-maltose + ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) US149 | alpha-maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67500, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
maltogenic amylase | - |
Bacillus sp. (in: Bacteria) |
MAUS149 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | after incubation for 2 h at 50°C, the wild type enzyme retains 35% of its initial activity. The wild type enzyme has a half-life of 15 min at 55°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
wild type enzyme | Bacillus sp. (in: Bacteria) |