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Literature summary for 3.2.1.133 extracted from
- Enzymatic synthesis and properties of highly branched rice starch amylose and amylopectin cluster (2008), J. Agric. Food Chem., 56, 126-131.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of highly branched amylopectin and amylose from enzymatically modified rice starch | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterogenous expression in Bacillus subtilis host strain ISW1214 | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Geobacillus stearothermophilus | - |
alpha-maltose + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | P19531 | maltogenic alpha-amylase precursor, amyM gene | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged protein purified via Ni-NTA colummns | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Geobacillus stearothermophilus | alpha-maltose + ? | - |
? | |
| starch + H2O | utilization of BSMA for production of highly branched amylopectin and amylose from enzymatically modified rice starch, branching by transglycosylation mediated by BSMA, increased number of branched side chains in modified amylopectin clusters determined | Geobacillus stearothermophilus | alpha-maltose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BSMA | - |
Geobacillus stearothermophilus |
| glucan 1,4-alpha-maltohydrolase | - |
Geobacillus stearothermophilus |
| maltogenic amylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
activity assay at | Geobacillus stearothermophilus |
| 55 | - |
synthesis reaction performed for 12 h using 500 units/g substrate for transgylcosylation | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at, 50 mM sodium citrate buffer | Geobacillus stearothermophilus |
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