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Literature summary for 3.2.1.133 extracted from
- Enzymatic characterization of a maltogenic amylase from Lactobacillus gasseri ATCC 33323 expressed in Escherichia coli (2005), FEMS Microbiol. Lett., 252, 175-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lactobacillus gasseri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | - |
Lactobacillus gasseri |  |
| Co2+ | 5 mM, 76% inhibition | Lactobacillus gasseri | |
| EDTA | 5 mM, 74% inhibition | Lactobacillus gasseri | |
| Fe2+ | 5 mM, 90% inhibition | Lactobacillus gasseri | |
| Zn2+ | 5 mM, 96% inhibition | Lactobacillus gasseri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Lactobacillus gasseri | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, causes 20% increase in hydrolysis of starch | Lactobacillus gasseri | |
| Mg2+ | 5 mM, causes 9% increase in hydrolysis of starch | Lactobacillus gasseri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
4 * 65000, SDS-PAGE | Lactobacillus gasseri |
| 211000 | - |
gel filtration | Lactobacillus gasseri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | - |
ATCC 33323 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Lactobacillus gasseri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 58.7 | - |
hydrolysis of beta-cyclodextrin | Lactobacillus gasseri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-cyclodextrin + H2O | relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 | Lactobacillus gasseri | maltose + ? | - |
? | |
| additional information | enzyme shows hydrolytic activity towards alpha-1,6-glycosidic linkage | Lactobacillus gasseri | ? | - |
? | |
| pullulan + H2O | relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 | Lactobacillus gasseri | maltose + D-glucose + panose | mainly maltose and glucose with relatively minor quantity of panose and other maltooligosaccharides | ? | |
| soluble starch + H2O | relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 | Lactobacillus gasseri | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 65000, SDS-PAGE | Lactobacillus gasseri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LGMA | - |
Lactobacillus gasseri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
hydrolysis of beta-cyclodextrin, starch and pullulan | Lactobacillus gasseri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 20% of maximal activity with starch, about 25% of maximal activity with beta-cyclodextrin and about 35% of maximal activity with pullulan, 60°C: about 40% of maximal activity with pullulan, about 50% of maximal activity with starch anf about 80% of maximal activity with beta-cyclodextrin | Lactobacillus gasseri |
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Release 2023.1 (January 2023)
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