Protein Variants | Comment | Organism |
---|---|---|
A398V | random mutagenesis, using DNA shuffling | Thermus sp. |
I333V | random mutagenesis, using DNA shuffling | Thermus sp. |
M375T | random mutagenesis, using DNA shuffling | Thermus sp. |
P453L | random mutagenesis, using DNA shuffling | Thermus sp. |
Q411L | random mutagenesis, using DNA shuffling | Thermus sp. |
R26Q | random mutagenesis, using DNA shuffling | Thermus sp. |
S169N | random mutagenesis, using DNA shuffling | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant DM | Thermus sp. | |
0.09 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 4B74 | Thermus sp. | |
0.16 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, wild-type and variants 3C71, 4B78 and 4A48 | Thermus sp. | |
0.17 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variants 1B76, 1B100 | Thermus sp. | |
0.19 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 2A39 | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Thermus sp. IM6501 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant DM | Thermus sp. |
0.3 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B74 | Thermus sp. |
1 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B78 | Thermus sp. |
1.5 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, wild-type | Thermus sp. |
1.7 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variants 2A39, 4A48 and 3C71 | Thermus sp. |
2 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 1B100 | Thermus sp. |
2.1 | - |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 1B76 | Thermus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type | Thermus sp. |
75 | - |
ThMA-DM, highly thermostable mutant enzyme | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
ThMA-DM, highly thermostable mutant enzyme, half-life 172 min, wild type enzyme completely inactivated in less than 1 min | Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variants DM and 4B74 | Thermus sp. | |
110 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 4B78 | Thermus sp. | |
120 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 3C71 | Thermus sp. | |
130 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 4A48 | Thermus sp. | |
160 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, wild-type | Thermus sp. | |
170 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 2A39 | Thermus sp. | |
190 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 1B100 | Thermus sp. | |
200 | - |
beta-cyclodextrin | pH 6.0, 60°C, hydrolysis, variant 1B76 | Thermus sp. |