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Literature summary for 3.2.1.132 extracted from

  • Zhang, J.; Cao, H.; Li, S.; Zhao, Y.; Wang, W.; Xu, Q.; Du, Y.; Yin, H.
    Characterization of a new family 75 chitosanase from Aspergillus sp. W-2 (2015), Int. J. Biol. Macromol., 81, 362-369 .
    View publication on PubMed
Search for more literature for 3.2.1.132

Application

Application Comment Organism
synthesis the enzyme can be a competitive candidate for chitosan oligosaccharide-manufacturing industry Aspergillus sp. W-2

Cloned(Commentary)

Cloned (Comment) Organism
the mature CsnW2 protein is fused to His-tag at C-terminus and expressed in Pichia pastoris X-33 Aspergillus sp. W-2

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 90% loss of activity Aspergillus sp. W-2
Fe2+ 1 mM, 39% loss of activity Aspergillus sp. W-2
Ge2+ 1 mM, 83% loss of activity Aspergillus sp. W-2
Ni2+ 1 mM, 84% loss of activity Aspergillus sp. W-2
Zn2+ 1 mM, 75% loss of activity Aspergillus sp. W-2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 chitosan Km-value: 7.1 mg/ml, pH 6.0, 55°C Aspergillus sp. W-2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.1fold activation Aspergillus sp. W-2
Mg2+ 1 mM, 1.1fold activation Aspergillus sp. W-2
Mn2+ 1 mM, 1.2fold activation Aspergillus sp. W-2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25148
-
 calculated from sequence Aspergillus sp. W-2

Organism

Organism UniProt Comment Textmining
Aspergillus sp. W-2 A0A0B5L773
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus sp. W-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + H2O (GlcN)6 is mainly hydrolyzed into two molecules of (GlcN)3, accompanied by a very small proportion of (GlcN)2 and (GlcN)4 Aspergillus sp. W-2 beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
-
 ?
chitosan + H2O the enzyme prefers to hydrolyze chitosan with high degree of deacetylation. The main products of chitiosan (92% deacetylation) are chitosan oligosaccharides with degree of polymerization (DP) ranging from 2 to 6. No activity with colloidal chitin, carboxymethylcellulose, microcrystalline cellulose, glucosan Aspergillus sp. W-2 chitosan oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
csnw2
-
 Aspergillus sp. W-2
endo-acting chitinase
-
 Aspergillus sp. W-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus sp. W-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 0.5 h, 16% loss of activity. 1 h, 22% loss of activity Aspergillus sp. W-2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
 chitosan pH 6.0, 55°C Aspergillus sp. W-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus sp. W-2

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 1 h, the enzyme retains over 79% of its activity Aspergillus sp. W-2