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Literature summary for 3.2.1.132 extracted from

  • Han, Y.; Gao, P.; Yu, W.; Lu, X.
    Thermostability enhancement of chitosanase CsnA by fusion a family 5 carbohydrate-binding module (2017), Biotechnol. Lett., 39, 1895-1901 .
    View publication on PubMed

Application

Application Comment Organism
industry the fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) exhibits higher chitosan binding capacity and catalytic activity than wild-type enzyme and improved thermostability. The hybrid enzyme is a useful candidate for industrial application Renibacterium sp. QD1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Renibacterium sp. QD1

Protein Variants

Protein Variants Comment Organism
additional information the fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) exhibits higher chitosan binding capacity and catalytic activity than wild-type enzyme. The fusion of BgCBM5 significantly improves the thermostability of citosanase CsnA Renibacterium sp. QD1

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 86.5% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 87% inhibition of wild-type enzyme Renibacterium sp. QD1
Cu2+ 1 mM, complete inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) and of wild-type enzyme Renibacterium sp. QD1
Fe3+ 1 mM, 11% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 13% inhibition of wild-type enzyme Renibacterium sp. QD1
Ni2+ 1 mM, 22% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM,10% inhibition of wild-type enzyme Renibacterium sp. QD1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
chitosan KM-value: 31.5 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35), pH 5.6, 55°C Renibacterium sp. QD1
additional information
-
chitosan KM-value: 42 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2), pH 5.6, 55°C Renibacterium sp. QD1
additional information
-
chitosan KM-value: 43 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), pH 5.6, 55°C Renibacterium sp. QD1
additional information
-
chitosan KM-value: 69 g/l, wild-type enzyme, pH 5.6, 55°C Renibacterium sp. QD1

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 1.5fold activation of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), 1.9fold activation of wild-type enzyme Renibacterium sp. QD1

Organism

Organism UniProt Comment Textmining
Renibacterium sp. QD1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Renibacterium sp. QD1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O colloidal chitosan Renibacterium sp. QD1 ?
-
?

Synonyms

Synonyms Comment Organism
chitosanase CsnA
-
Renibacterium sp. QD1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
wild-type enzyme and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) Renibacterium sp. QD1
55
-
fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) Renibacterium sp. QD1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.9
-
T50-value, wild-type enzyme Renibacterium sp. QD1
57.8
-
T50-value, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) Renibacterium sp. QD1
65
-
1 h, wild-type enzyme and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) retain 37% activity. Complete loss of activity of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) Renibacterium sp. QD1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20800
-
chitosan fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35), pH 5.6, 55°C Renibacterium sp. QD1
30100
-
chitosan fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2), pH 5.6, 55°C Renibacterium sp. QD1
64400
-
chitosan fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), pH 5.6, 55°C Renibacterium sp. QD1
72000
-
chitosan wild-type enzyme, pH 5.6, 55°C Renibacterium sp. QD1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
wild-type enzyme, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) Renibacterium sp. QD1