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Literature summary for 3.2.1.132 extracted from

  • Zhou, Z.; Zhao, S.; Wang, S.; Li, X.; Su, L.; Ma, Y.; Li, J.; Song, J.
    Extracellular overexpression of chitosanase from Bacillus sp. TS in Escherichia coli (2015), Appl. Biochem. Biotechnol., 175, 3271-3286 .
    View publication on PubMed
Search for more literature for 3.2.1.132

Application

Application Comment Organism
industry the high yield of the extracellular overexpression, relevant thermostability, and effective hydrolysis of commercial grade chitosan shows that this recombinant enzyme has a great potential for industrial applications Bacillus sp. TS

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Bacillus sp. TS

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.5 mM, about 60% loss of activity Bacillus sp. TS
Cu2+ 1 mM, complete loss of activity Bacillus sp. TS
Hg2+ 1 mM, about 75% loss of activity Bacillus sp. TS

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM, about 1.5fold activation Bacillus sp. TS

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 molecular mass of recombinant chitosanase with His6-tag at the C-terminus, SDS-PAGE Bacillus sp. TS

Organism

Organism UniProt Comment Textmining
Bacillus sp. TS A0A167KLN5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. TS

Source Tissue

Source Tissue Comment Organism Textmining
culture condition the highest level of enzyme activity (186 U/ml) is achieved in culture medium using high cell-density cultivation in a 7-l fermenter. Chitosanase is successfully secreted to the culture media through the widely used SecB-dependent type II pathway in Escherichia coli Bacillus sp. TS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O the main products of chitosan hydrolyzed by recombinant chitosanase are (GlcN)3-6 Bacillus sp. TS chitotriose + chitotetraose + chitopentaose + chitohexaose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. TS

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 50% of maximal activity, 70°C: about 60% of maximal activity Bacillus sp. TS

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 24 h, enzyme retains 96.5% of its initial activity Bacillus sp. TS
40
-
 24 h, enzyme retains 77.9% of its initial activity Bacillus sp. TS
45
-
 half-life time: 66 min Bacillus sp. TS
60
-
 the enzyme loses activity within 10 min at 60°C Bacillus sp. TS

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus sp. TS

pH Range

pH Minimum pH Maximum Comment Organism
4.6 6 pH 4.6: about 60% of maximal activity, pH 6.0: about 50% of maximal activity Bacillus sp. TS