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Literature summary for 3.2.1.132 extracted from

  • Struszczyk, K.; Szczesna-Antczak, M.; Walczak, M.; Pomianowska, E.; Antczak, T.
    Isolation and purification of Mucor circinelloides intracellular chitosanolytic enzymes (2009), Carbohydr. Polym., 78, 16-24.
    View publication on PubMed
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 89% inhibition at 1 mM, 79% at 0.5 mM Mucor circinelloides
Ba2+ 11% inhibition at 1 mM Mucor circinelloides
Cd2+ 13% inhibition at 1 mM Mucor circinelloides
Co2+ 13% inhibition at 1 mM Mucor circinelloides
Cu2+ 92% inhibition at 1 mM, 82% at 0.5 mM Mucor circinelloides
EDTA 17% inhibition at 1% w/v, 82% at 5% w/v Mucor circinelloides
Fe2+ 26% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides
Hg2+ complete inhibition at 1 mM Mucor circinelloides
Pb2+ 14% inhibition at 1 mM, 8% at 0.5 mM Mucor circinelloides
SDS 82% inhibition at 1% w/v Mucor circinelloides
Sn2+ 20% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides
Triton X-100 80% inhibition at 1% w/v Mucor circinelloides
Tween 20 72% inhibition at 1% w/v Mucor circinelloides
Tween 80 79% inhibition at 1% w/v Mucor circinelloides
Zn2+ 24% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Mucor circinelloides 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Mucor circinelloides
Mg2+ activates Mucor circinelloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Mucor circinelloides

Organism

Organism UniProt Comment Textmining
Mucor circinelloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 23fold from mycelium by bacitracin affinity chromatography and gel filtration Mucor circinelloides

Source Tissue

Source Tissue Comment Organism Textmining
mycelium air-dried and defatted Mucor circinelloides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O the chitosanase is an endo-type enzyme, the preferred substrates of chitosanase are chitosan preparations with a high degree of deacetylation, substrate is chitosan 66-97% deacetylated Mucor circinelloides ?
-
 ?
additional information The enzyme might be identical with a lipase that co-purifies with the chitosanase. No activity for colloidal chitin, Na-CMC and starch Mucor circinelloides ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Mucor circinelloides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mucor circinelloides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 15-20% of maximal activity at 20°C, 30% at 50°C, temperature profile, overview Mucor circinelloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Mucor circinelloides

pH Range

pH Minimum pH Maximum Comment Organism
4 7 20% of maximal activity at pH 4.0, 10% at pH 7.0, pH profile, overview Mucor circinelloides