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Literature summary for 3.2.1.132 extracted from

  • Lee, Y.S.; Yoo, J.S.; Chung, S.Y.; Lee, Y.C.; Cho, Y.S.; Choi, Y.L.
    Cloning, purification, and characterization of chitosanase from Bacillus sp. DAU101 (2006), Appl. Microbiol. Biotechnol., 73, 113-121.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus sp. DAU101

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Bacillus sp. DAU101
Ni(2+)
-
Bacillus sp. DAU101

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5-10 mM, activity is increased by about 1.6fold Bacillus sp. DAU101

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, SDS-PAGE Bacillus sp. DAU101
31420
-
x * 31420, calculated from sequence Bacillus sp. DAU101

Organism

Organism UniProt Comment Textmining
Bacillus sp. DAU101
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification deletion of a signal peptide with SDS-PAGE Bacillus sp. DAU101

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. DAU101

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
Bacillus sp. DAU101 chitobiose + chitotriose + chitotetraose
-
?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Bacillus sp. DAU101
? x * 31420, calculated from sequence Bacillus sp. DAU101

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. DAU101

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Bacillus sp. DAU101