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Literature summary for 3.2.1.132 extracted from

  • Choi, Y.J.; Kim, E.J.; Piao, Z.; Yun, Y.C.; Shin, Y.C.
    Purification and characterization of chitosanase from Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides (2004), Appl. Environ. Microbiol., 70, 4522-4531.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.132

Application

Application Comment Organism
synthesis valuable enzyme for the commercial production of chitosan oligosaccharides and other chitosan hydrolysates Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, 68% inhibition Bacillus sp. (in: Bacteria)
KCl 0.4 M, 50% inhibition Bacillus sp. (in: Bacteria)
Mn2+ 1 mM, 69% inhibition Bacillus sp. (in: Bacteria)
NaCl 0.4 M, 50% inhibition Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q9ALZ1
-
-
Bacillus sp. (in: Bacteria) KCTC 0377BP Q9ALZ1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1700
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitoheptaose + H2O 23.8% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
chitoheptaose + H2O 23.8% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
chitohexaose + H2O 20.0% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
chitopentaose + H2O 16.8% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
chitopentaose + H2O 16.8% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
chitosan + H2O activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate Bacillus sp. (in: Bacteria) chitosan oligosaccharides
-
 ?
chitosan + H2O activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate Bacillus sp. (in: Bacteria) KCTC 0377BP chitosan oligosaccharides
-
 ?
chitotetraose + H2O 15.0% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
chitotetraose + H2O 15.0% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
additional information no hydrolysis of chitobiose and chitotriose Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no hydrolysis of chitobiose and chitotriose Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 24 h, enzyme retains full activity Bacillus sp. (in: Bacteria)
50
-
 half-life: 5 h Bacillus sp. (in: Bacteria)
55
-
 half-life: 10 min Bacillus sp. (in: Bacteria)
60
-
 half-life: 5 min Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 1 h, complete inactivation Bacillus sp. (in: Bacteria)
4 8 1 h, stable Bacillus sp. (in: Bacteria)