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Literature summary for 3.2.1.132 extracted from
- Purification and properties of chitosanase from Bacillus circulans MH-K1 (1988), J. Gen. Appl. Microbiol., 34, 255-270.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Niallia circulans |  |
| Hg2+ | - |
Niallia circulans | |
| Ni2+ | - |
Niallia circulans | |
| p-chloromercuribenzoate | - |
Niallia circulans | |
| Zn2+ | - |
Niallia circulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.63 mg chitosan/ml | Niallia circulans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Niallia circulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Niallia circulans |
| 32000 | - |
1 * 32000, SDS-PAGE | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Niallia circulans | - |
? | - |
? | |
| chitosan + H2O | Niallia circulans MH-K1 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
MH-K1 | - |
| Niallia circulans MH-K1 | - |
MH-K1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Niallia circulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Niallia circulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Niallia circulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
after gel filtration | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylchitosan + H2O | - |
Niallia circulans | ? | - |
? | |
| chitosan + H2O | - |
Niallia circulans | ? | - |
? | |
| chitosan + H2O | - |
Niallia circulans MH-K1 | ? | - |
? | |
| chitosan + H2O | the smallest of the substrates is a tetramer of glucosamine | Niallia circulans | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | substrates with less than 40% deacetylation are not affected by the enzyme | Niallia circulans | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | most susceptible substrate is: 80% deacetylated chitosan | Niallia circulans | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | hydrolysis of: glycol chitosan | Niallia circulans | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | the smallest of the substrates is a tetramer of glucosamine | Niallia circulans MH-K1 | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | substrates with less than 40% deacetylation are not affected by the enzyme | Niallia circulans MH-K1 | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | most susceptible substrate is: 80% deacetylated chitosan | Niallia circulans MH-K1 | additional information | mixture of dimer and trimer of glucosamine | ? | |
| additional information | no activity with chitin (i.e. 100% acetylated chitosan) | Niallia circulans | ? | - |
? | |
| additional information | no activity with chitin (i.e. 100% acetylated chitosan) | Niallia circulans MH-K1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at pH 6.5 | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
stable in this range | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | about 20% of maximal activity at pH 4 and 8 | Niallia circulans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Niallia circulans | density gradient column electrofocusing and acrylamide gel electrofocusing | - |
9.2 |
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