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Literature summary for 3.2.1.132 extracted from

  • Tominaga, Y.; Tsujisaka, Y.
    Purification and some enzymatic properties of the chitosanase from Bacillus R-4 which lyses Rhizopus cell walls (1975), Biochim. Biophys. Acta, 410, 145-155.
    View publication on PubMed
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
Chelating agents especially EDTA, activate Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)
L-cysteine
-
 Bacillus sp. (in: Bacteria)
monoiodoacetate
-
 Bacillus sp. (in: Bacteria)
Ni2+
-
 Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate
-
 Bacillus sp. (in: Bacteria)
reduced glutathione
-
 Bacillus sp. (in: Bacteria)
sulfhydryl reagents glutathione or L-cysteine restores activity Bacillus sp. (in: Bacteria)
Zn2+
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) R-4
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) K-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 K-1
-
Bacillus sp. (in: Bacteria)
-
 R-4
-
Bacillus sp. (in: Bacteria) K-1
-
 K-1
-
Bacillus sp. (in: Bacteria) R-4
-
 R-4
-

Purification (Commentary)

Purification (Comment) Organism
R-4 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.61
-
 after Sephadex G-75 chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) R-4 ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) additional information
-
 ?
chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) R-4 additional information
-
 ?
chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) K-1 additional information
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable Bacillus sp. (in: Bacteria)
70
-
 15 min, 30% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing
-
 8.3