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Literature summary for 3.2.1.131 extracted from
- A novel family of hemicellulolytic alpha-glucuronidase (2009), FEBS Lett., 583, 1457-1462.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | alpha-glucuronidase due to its catalytic properties may be of great importance in processes of enzymatic biomass conversion as well as a tool for altering rheological properties of glucuronoxylans | Scheffersomyces stipitis CBS 6054 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
x * 120000, SDS-PAGE | Scheffersomyces stipitis CBS 6054 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scheffersomyces stipitis CBS 6054 | A3LY17 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from a concentrated induction medium (mixture of xylobiose, xylotriose and methyl beta-D-xylopyranoside) by a combination of ion-exchange and hydrophobic interaction chromatographies, 9.2fold | Scheffersomyces stipitis CBS 6054 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.67 | - |
concentrated induction medium | Scheffersomyces stipitis CBS 6054 |
| 6.2 | - |
9.2fold purified enzyme | Scheffersomyces stipitis CBS 6054 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldopentaouronic acid + H2O | - |
Scheffersomyces stipitis CBS 6054 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? |  |
| aldotetraouronic acid + H2O | - |
Scheffersomyces stipitis CBS 6054 | 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
| beech glucuronoxylan + H2O | - |
Scheffersomyces stipitis CBS 6054 | 4-O-methyl-D-glucuronic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000, SDS-PAGE | Scheffersomyces stipitis CBS 6054 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucuronidase | - |
Scheffersomyces stipitis CBS 6054 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Scheffersomyces stipitis CBS 6054 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
is stable for at least 3 h | Scheffersomyces stipitis CBS 6054 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Scheffersomyces stipitis CBS 6054 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.5 | at pH 4.0 and pH 5.5 the activities represent 25.5% and 51.6% of the activity at the optimum pH, respectively | Scheffersomyces stipitis CBS 6054 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Scheffersomyces stipitis CBS 6054 | isoelectrofocusing | - |
4 |
| Scheffersomyces stipitis CBS 6054 | sequence analysis | - |
4.64 |
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Release 2023.1 (January 2023)
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