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Literature summary for 3.2.1.130 extracted from

  • Thompson, A.J.; Williams, R.J.; Hakki, Z.; Alonzi, D.S.; Wennekes, T.; Gloster, T.M.; Songsrirote, K.; Thomas-Oates, J.E.; Wrodnigg, T.M.; Spreitz, J.; Stuetz, A.E.; Butters, T.D.; Williams, S.J.; Davies, G.J.
    Structural and mechanistic insight into N-glycan processing by endo-alpha-mannosidase (2012), Proc. Natl. Acad. Sci. USA, 109, 781-786.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.130

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacteroides xylanisolvens
expressed in Escherichia coli BL21(DE3) cells Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment) Organism
single wavelength anomalous dispersion technique Bacteroides xylanisolvens
single wavelength anomalous dispersion technique Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
E154A the mutant shows near zero activity Bacteroides thetaiotaomicron
E329A the mutant shows near zero activity Bacteroides thetaiotaomicron
E332A the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme Bacteroides thetaiotaomicron

Inhibitors

Inhibitors Comment Organism Structure
(3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine
-
 Bacteroides thetaiotaomicron
(3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine
-
 Bacteroides xylanisolvens
alpha-glucopyranosyl-(1->3)-deoxymannojirimycin
-
 Bacteroides thetaiotaomicron
alpha-glucopyranosyl-(1->3)-deoxymannojirimycin
-
 Bacteroides xylanisolvens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
0.7
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens
2.6
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcMan7GlcNAc2 + H2O Bacteroides thetaiotaomicron
-
 Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O Bacteroides xylanisolvens
-
 Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O Bacteroides thetaiotaomicron DSM 2079
-
 Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O Bacteroides xylanisolvens XB1A
-
 Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
 ?
GlcMan9GlcNAc2 + H2O Bacteroides xylanisolvens
-
 ?
-
 ?
GlcMan9GlcNAc2 + H2O Bacteroides thetaiotaomicron
-
 ?
-
 ?
GlcMan9GlcNAc2 + H2O Bacteroides thetaiotaomicron DSM 2079
-
 ?
-
 ?
GlcMan9GlcNAc2 + H2O Bacteroides xylanisolvens XB1A
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A109
-
-
Bacteroides thetaiotaomicron DSM 2079 Q8A109
-
-
Bacteroides xylanisolvens D6D1V7
-
-
Bacteroides xylanisolvens XB1A D6D1V7
-
-

Purification (Commentary)

Purification (Comment) Organism
NiSO4-charged HiTrap column chromatography and S75 gel filtration Bacteroides xylanisolvens
NiSO4-charged HiTrap column chromatography and S75 gel filtration Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
-
 Bacteroides xylanisolvens XB1A ?
-
 ?
Glc(1->3)Man9GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron Man8GlcNAc + glucosyl alpha(1->3)-mannose
-
 ?
Glc(1->3)Man9GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 Man8GlcNAc + glucosyl alpha(1->3)-mannose
-
 ?
Glc(1->3)Man9GlcNAc2 + H2O
-
 Bacteroides xylanisolvens Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
Glc(1->3)Man9GlcNAc2 + H2O
-
 Bacteroides xylanisolvens XB1A Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan7GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O
-
 Bacteroides xylanisolvens Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
 ?
GlcMan7GlcNAc2 + H2O
-
 Bacteroides xylanisolvens XB1A Man6GlcNAc2 + glucosyl alpha-(1->3)-D-mannose
-
 ?
GlcMan9GlcNAc2 + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
GlcMan9GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
GlcMan9GlcNAc2 + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?
GlcMan9GlcNAc2 + H2O
-
 Bacteroides xylanisolvens XB1A ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-alpha-mannosidase
-
 Bacteroides xylanisolvens
endo-alpha-mannosidase
-
 Bacteroides thetaiotaomicron
GH99
-
 Bacteroides xylanisolvens
GH99
-
 Bacteroides thetaiotaomicron

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
4.77
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
6.34
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride mutant enzyme E332A, at pH 8.0 and 37°C Bacteroides thetaiotaomicron
9.1
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides xylanisolvens
9.9
-
 alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride wild type enzyme, at pH 8.0 and 37°C Bacteroides thetaiotaomicron