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Literature summary for 3.2.1.129 extracted from

  • Haile, Y.; Berski, S.; Draeger, G.; Nobre, A.; Stummeyer, K.; Gerardy-Schahn, R.; Grothe, C.
    The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells (2008), Biomaterials, 29, 1880-1891.
    View publication on PubMed
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Application

Application Comment Organism
biotechnology degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats Escherichia phage K1F
degradation degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats Escherichia phage K1F
medicine degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering: no degradation in 12 days with 1 microg/ml active enzyme + 105 cubic mm hydrogel in phosphate buffered saline (pH 7.4), at room temperature, increase to 4 microg/ml at end of week 2 initiates degradation, total degradation after 4 weeks, hydrogel was coated with poly-L-lysine, poly-L-ornithine-laminin or collagen for neurite formation in neonatal and adult rat Schwann cells, neural rat stem cells, and dorsal root ganglionic cells from rats Escherichia phage K1F

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-2,8-linked polysialic acid + H2O Escherichia phage K1F specifically cleaves alpha 2,8-linked sialic acid residues with minimum chain length of 8, no effect on other sialic acid containing structures ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F
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Source Tissue

Source Tissue Comment Organism Textmining
additional information neonatal rat Schwann cell culture (1-3 days postnatal), adult rat Schwann cell culture from 3 months old females, neural stem cells of fetal rat cell culture (gestational day 12), dorsal ganglionic cells of neonatal rat cell culture (2-3 days postnatal) Escherichia phage K1F
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-2,8-linked polysialic acid + H2O specifically cleaves alpha 2,8-linked sialic acid residues with minimum chain length of 8, no effect on other sialic acid containing structures Escherichia phage K1F ?
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 ?
alpha-2,8-linked sialic acid residues cross-linked by diepoxyoctane + H2O hydrogel with minimum chain length of 8 Escherichia phage K1F ?
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 ?

Synonyms

Synonyms Comment Organism
endo-N 1588
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 Escherichia phage K1F
endosialidase
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 Escherichia phage K1F