Application | Comment | Organism |
---|---|---|
additional information | the C-terminal domain plays a crucial role in folding and assembling not only the C-terminal domain of endosialidases but also of other, unrelated phage proteins | Escherichia phage K1F |
Cloned (Comment) | Organism |
---|---|
subcloned into the NdeI and XhoI restriction sites of pET22b, resulting in a construct encoding the C-terminal domain of endoNF with a C-terminal His6 tag, expressed in Escherichia coli BL21-Gold(DE3) | Escherichia phage K1F |
Protein Variants | Comment | Organism |
---|---|---|
G956A | completely loses enzymatic activity | Escherichia phage K1F |
H954A | completely loses enzymatic activity | Escherichia phage K1F |
additional information | mutant deltaN-endoNF lacking the capsid binding domain, forms trimeric complexes | Escherichia phage K1F |
N912A | completely loses enzymatic activity | Escherichia phage K1F |
R1035A | completely loses enzymatic activity | Escherichia phage K1F |
S911A | mutant deltaN-endoNF lacking the capsid binding domain but retaining the C-terminal domain, prevents cleavage but not assembly into active trimers | Escherichia phage K1F |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, affinity chromatography and a Ni2+-chelating column | Escherichia phage K1F |
Subunits | Comment | Organism |
---|---|---|
trimer | gel filtration | Escherichia phage K1F |
Synonyms | Comment | Organism |
---|---|---|
endo-N-acetylneuraminidase | - |
Escherichia phage K1F |
endoNF | - |
Escherichia phage K1F |
endosialidase | - |
Escherichia phage K1F |