Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Escherichia phage K1F |
Crystallization (Comment) | Organism |
---|---|
in complex with oligomeric sialic acid, 1.9 A resolution | Escherichia phage K1F |
Protein Variants | Comment | Organism |
---|---|---|
E581A | behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation | Escherichia phage K1F |
R547A/E581A | inactive, but no effect on expression, maturation or comlex formation | Escherichia phage K1F |
R596A | behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation | Escherichia phage K1F |
R596A/E581A | inactive, but no effect on expression, maturation or comlex formation | Escherichia phage K1F |
R596A/R647A | inactive, but no effect on expression, maturation or comlex formation | Escherichia phage K1F |
R647A | behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation | Escherichia phage K1F |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | Q04830 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Escherichia phage K1F |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-2,8-glycosidically linked sialic acid + H2O | 37°C | Escherichia phage K1F | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | crystal structure | Escherichia phage K1F |
Synonyms | Comment | Organism |
---|---|---|
endosialidase NF | - |
Escherichia phage K1F |