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Literature summary for 3.2.1.129 extracted from

  • Stummeyer, K.; Dickmanns, A.; Mühlenhoff, M.; Gerardy-Schahn, R.; Ficner, R.
    Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F (2005), Nat. Struct. Mol. Biol., 12, 90-96.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia phage K1F

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with oligomeric sialic acid, 1.9 A resolution Escherichia phage K1F

Protein Variants

Protein Variants Comment Organism
E581A behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation Escherichia phage K1F
R547A/E581A inactive, but no effect on expression, maturation or comlex formation Escherichia phage K1F
R596A behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation Escherichia phage K1F
R596A/E581A inactive, but no effect on expression, maturation or comlex formation Escherichia phage K1F
R596A/R647A inactive, but no effect on expression, maturation or comlex formation Escherichia phage K1F
R647A behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation Escherichia phage K1F

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F Q04830
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Escherichia phage K1F

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-2,8-glycosidically linked sialic acid + H2O 37°C Escherichia phage K1F ?
-
?

Subunits

Subunits Comment Organism
trimer crystal structure Escherichia phage K1F

Synonyms

Synonyms Comment Organism
endosialidase NF
-
Escherichia phage K1F