BRENDA - Enzyme Database show
show all sequences of 3.2.1.126

Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides

Escamilla-Trevino, L.L.; Chen, W.; Card, M.L.; Shih, M.C.; Cheng, C.L.; Poulton, J.E.; Phytochemistry 67, 1651-1660 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cDNAs encoding BGLU45 and BGLU46 expressed in Pichia pastoris
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
p-coumaryl beta-D-glucoside
BGLU46
Arabidopsis thaliana
5.1
-
syringin
BGLU45
Arabidopsis thaliana
7
-
coniferin
BGLU45
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
ecotype Columbia
-
Purification (Commentary)
Commentary
Organism
to apparent homogeneity by ammonium sulfate fractionation and hydrophobic interaction chromatography, BGLU45 8.8fold and BGLU46 13.7fold purified
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
BGLU45 and BGLU46 absent from rosette leaves or flowers
Arabidopsis thaliana
-
root
BGLU46
Arabidopsis thaliana
-
silique
BGLU45
Arabidopsis thaliana
-
stem
BGLU45 and BGLU46 display increasing levels of expression from apex to base, matching the known increase in lignification
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1
-
BGLU45, crude extract
Arabidopsis thaliana
35.9
-
BGLU45, purified enzyme
Arabidopsis thaliana
43.8
-
BGLU46, crude extract
Arabidopsis thaliana
599
-
BGLU46, purified enzyme
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-amygdalin
-
682290
Arabidopsis thaliana
?
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside
9% activity with BGLU46
682290
Arabidopsis thaliana
4-methylumbelliferol + beta-D-glucose
-
-
-
?
arbutin
6% activity with BGLU46
682290
Arabidopsis thaliana
benzene-1,4-diol + beta-D-glucose
-
-
-
?
coniferin
87% activity with BGLU45 and 8% activity with BGLU46
682290
Arabidopsis thaliana
beta-D-glucose + coniferyl aldehyde
-
-
-
?
additional information
no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside
682290
Arabidopsis thaliana
?
-
-
-
-
o-nitrophenyl-beta-D-glucopyranoside
most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation
682290
Arabidopsis thaliana
o-nitrophenol + beta-D-glucose
-
-
-
-
p-coumaryl beta-D-glucoside
6.9% activity with BGLU45 and 71% activity with BGLU46
682290
Arabidopsis thaliana
p-coumaric acid + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside
0.4% activity with BGLU45 and 21% activity with BGLU46
682290
Arabidopsis thaliana
p-nitrophenol + beta-D-galactose
-
-
-
-
p-nitrophenyl-beta-D-glucopyranoside
100% activity with BGLU45 and BGLU46
682290
Arabidopsis thaliana
p-nitrophenol + beta-D-glucose
-
-
-
-
phenyl-beta-D-glucoside
62% activity with BGLU46
682290
Arabidopsis thaliana
phenol + beta-D-glucose
-
-
-
?
salicin
100% activity with BGLU46
682290
Arabidopsis thaliana
beta-D-glucose + salicyl alcohol
-
-
-
?
syringin + H2O
100% activity with BGLU45 and 6% activity with BGLU46
682290
Arabidopsis thaliana
D-glucose + sinapyl alcohol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNAs encoding BGLU45 and BGLU46 expressed in Pichia pastoris
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
p-coumaryl beta-D-glucoside
BGLU46
Arabidopsis thaliana
5.1
-
syringin
BGLU45
Arabidopsis thaliana
7
-
coniferin
BGLU45
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
to apparent homogeneity by ammonium sulfate fractionation and hydrophobic interaction chromatography, BGLU45 8.8fold and BGLU46 13.7fold purified
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
BGLU45 and BGLU46 absent from rosette leaves or flowers
Arabidopsis thaliana
-
root
BGLU46
Arabidopsis thaliana
-
silique
BGLU45
Arabidopsis thaliana
-
stem
BGLU45 and BGLU46 display increasing levels of expression from apex to base, matching the known increase in lignification
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1
-
BGLU45, crude extract
Arabidopsis thaliana
35.9
-
BGLU45, purified enzyme
Arabidopsis thaliana
43.8
-
BGLU46, crude extract
Arabidopsis thaliana
599
-
BGLU46, purified enzyme
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-amygdalin
-
682290
Arabidopsis thaliana
?
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside
9% activity with BGLU46
682290
Arabidopsis thaliana
4-methylumbelliferol + beta-D-glucose
-
-
-
?
arbutin
6% activity with BGLU46
682290
Arabidopsis thaliana
benzene-1,4-diol + beta-D-glucose
-
-
-
?
coniferin
87% activity with BGLU45 and 8% activity with BGLU46
682290
Arabidopsis thaliana
beta-D-glucose + coniferyl aldehyde
-
-
-
?
additional information
no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside
682290
Arabidopsis thaliana
?
-
-
-
-
o-nitrophenyl-beta-D-glucopyranoside
most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation
682290
Arabidopsis thaliana
o-nitrophenol + beta-D-glucose
-
-
-
-
p-coumaryl beta-D-glucoside
6.9% activity with BGLU45 and 71% activity with BGLU46
682290
Arabidopsis thaliana
p-coumaric acid + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside
0.4% activity with BGLU45 and 21% activity with BGLU46
682290
Arabidopsis thaliana
p-nitrophenol + beta-D-galactose
-
-
-
-
p-nitrophenyl-beta-D-glucopyranoside
100% activity with BGLU45 and BGLU46
682290
Arabidopsis thaliana
p-nitrophenol + beta-D-glucose
-
-
-
-
phenyl-beta-D-glucoside
62% activity with BGLU46
682290
Arabidopsis thaliana
phenol + beta-D-glucose
-
-
-
?
salicin
100% activity with BGLU46
682290
Arabidopsis thaliana
beta-D-glucose + salicyl alcohol
-
-
-
?
syringin + H2O
100% activity with BGLU45 and 6% activity with BGLU46
682290
Arabidopsis thaliana
D-glucose + sinapyl alcohol
-
-
-
?
Other publictions for EC 3.2.1.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751886
Baiya
Demonstration of monolignol b ...
Oryza sativa Japonica Group
Plant Physiol. Biochem.
127
223-230
2018
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751432
Tsuyama
-
Coniferin beta-glucosidase is ...
Populus sieboldii x Populus grandidentata
J. Wood Sci.
61
438-444
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682290
Escamilla-Trevino
Arabidopsis thaliana beta-gluc ...
Arabidopsis thaliana
Phytochemistry
67
1651-1660
2006
-
-
1
-
-
-
-
3
-
-
-
-
-
6
-
-
1
-
-
4
4
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
4
4
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650284
Watt
Agrobacterium tumefaciens beta ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1385
78-88
1998
6
-
1
-
-
-
5
10
-
-
2
1
-
1
-
-
1
1
-
-
1
-
12
1
1
-
-
11
1
-
-
-
4
1
-
6
-
1
-
-
-
-
-
5
4
10
-
-
2
1
-
-
-
1
-
-
1
-
12
1
1
-
-
11
1
-
-
1
-
-
-
-
-
-
653495
Dharmawardhana
A beta-glucosidase from lodgep ...
Pinus contorta
Plant Physiol.
107
331-339
1995
-
-
-
-
-
-
2
4
-
-
2
1
-
3
-
1
1
-
-
1
1
-
8
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
4
-
-
2
1
-
-
1
1
-
1
1
-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
26044
Leinhos
Purification of an acidic coni ...
Pinus banksiana
Phytochemistry
37
311-315
1994
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
1
1
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
1
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
26042
Hösel
-
Relationship of coniferin beta ...
Petroselinum crispum, Triticum aestivum
Plant Cell Tissue Organ Cult.
1
137-148
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
26041
Hösel
Characterization of beta-gluco ...
Cicer arietinum
Eur. J. Biochem.
84
487-492
1978
-
-
-
-
-
1
-
7
1
-
3
-
-
1
-
-
1
-
-
1
1
1
12
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
7
1
-
3
-
-
-
-
1
-
1
1
1
12
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
26043
Marcinowski
Enzymology of lignification. C ...
Picea abies
Eur. J. Biochem.
87
37-44
1978
-
-
-
-
-
-
-
7
1
-
2
1
-
1
-
-
1
-
-
3
1
2
13
1
2
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
1
-
2
1
-
-
-
1
-
3
1
2
13
1
2
-
-
-
2
1
-
-
-
-
-
-
-
-