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Literature summary for 3.2.1.126 extracted from

  • Dharmawardhana, D.P.; Ellis, B.E.; Carlson, J.E.
    A beta-glucosidase from lodgepole pine xylem specific for the lignin precursor coniferin (1995), Plant Physiol., 107, 331-339.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.126

Inhibitors

Inhibitors Comment Organism Structure
glucono-1,5-lactone
-
 Pinus contorta
additional information not inhibited by bromoconduritol or conduritol B epoxide Pinus contorta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 coniferin pH 5.5, 30°C Pinus contorta
0.29
-
 syringin pH 5.5, 30°C Pinus contorta
1.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C Pinus contorta
2.3
-
 4-methylumbelliferyl beta-D-glucoside pH 5.5, 30°C Pinus contorta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Pinus contorta
60000
-
 gel filtration, native PAGE Pinus contorta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferin + H2O Pinus contorta native substrate, may play a crucial role in stem lignification D-glucose + coniferol
-
 ?

Organism

Organism UniProt Comment Textmining
Pinus contorta
-
 var. latifolia Engelm, 10-15 years old
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Pinus contorta

Purification (Commentary)

Purification (Comment) Organism
2760fold Pinus contorta

Source Tissue

Source Tissue Comment Organism Textmining
xylem exclusive presence in the differentiating xylem Pinus contorta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
 pH 5.5, 30°C Pinus contorta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucoside + H2O 57% of the activity with coniferin Pinus contorta 2-nitrophenol + beta-D-glucose
-
 ?
4-methylumbelliferyl beta-D-glucoside + H2O 16% of the activity with coniferin Pinus contorta 4-methylumbelliferone + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O 26% of the activity with coniferin Pinus contorta 4-nitrophenol + beta-D-glucose
-
 ?
5-[4-(beta-D-glucopyranosyloxy)-3-methoxyphenylmethylene]-2-thioxothiazolidin-4-one-3-ethanoic acid + H2O synthetic coniferin analog, 45% of the activity with coniferin Pinus contorta ?
-
 ?
coniferin + H2O the native substrate is efficiently hydrolyzed, preferred substrates Pinus contorta D-glucose + coniferol
-
 ?
coniferin + H2O native substrate, may play a crucial role in stem lignification Pinus contorta D-glucose + coniferol
-
 ?
additional information not: disaccharide-containing glycoside, 4-nitrophenyl beta-cellobioside, 4-methylumbelliferyl alpha-glucoside Pinus contorta ?
-
 ?
syringin + H2O 51% of the activity with coniferin Pinus contorta D-glucose + sinapyl alcohol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Pinus contorta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pinus contorta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 5.9 optimum in the range at 30°C Pinus contorta

pI Value

Organism Comment pI Value Maximum pI Value
Pinus contorta chromatofocussing, pH gradient of 5.5 to 4
-
 4.5