KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
coniferin | roots | Picea abies | |
0.6 | - |
Picein | 2-nitrophenyl beta-D-glucoside, 2-nitrophenyl beta-D-galactoside, hypocotyls | Picea abies | |
0.7 | - |
4-nitrophenyl beta-D-glucoside | hypocotyls | Picea abies | |
1 | - |
4-methylumbelliferyl beta-D-glucoside | hypocotyls | Picea abies | |
1.3 | - |
coniferin | hypocotyls | Picea abies | |
1.8 | - |
syringin | hypocotyls | Picea abies | |
6.3 | - |
Picein | hypocotyls | Picea abies |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Picea abies | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
1 * 57000, Picea abies, SDS-PAGE | Picea abies |
58570 | - |
Picea abies, sedimentation equilibrium method | Picea abies |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferin + H2O | Picea abies | plant cell wall enzyme, involved in lignin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | - |
spruce | - |
Purification (Comment) | Organism |
---|---|
hypocotyls | Picea abies |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypocotyl | - |
Picea abies | - |
root | - |
Picea abies | - |
seedling | tissue distribution | Picea abies | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
hypocotyls | Picea abies |
Storage Stability | Organism |
---|---|
-20°C, crude cell wall preparation, frozen with liquid nitrogen, at least 3 months | Picea abies |
4°C, in 0.025 M McIlvaine buffer, pH 5, 0.1 M NaCl, 0.02% NaN3, less than 10% loss of activity within 15 days | Picea abies |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactoside + H2O | reaction at 16% (isozyme I) or 13% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | 2-nitrophenol + D-galactose | - |
? | |
2-nitrophenyl beta-D-glucoside + H2O | best substrate | Picea abies | 2-nitrophenol + D-glucose | - |
? | |
2-nitrophenyl beta-D-xyloside + H2O | poor substrate | Picea abies | 2-nitrophenol + D-xylose | - |
? | |
4-coumaryl beta-D-glucoside + H2O | reaction at 25% (isozyme II) or 4% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | coumaric acid + D-glucose | - |
? | |
4-methylumbelliferyl beta-D-glucoside + H2O | reaction at 79% (isozyme II) or 51% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | 4-methylumbelliferone + D-glucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | poor substrate, not | Picea abies | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | reaction at 56% (isozyme II) or 8% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | 4-nitrophenol + D-glucose | - |
? | |
coniferin + H2O | reaction at 52% (isozyme I) or 38% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | coniferol + D-glucose | - |
? | |
coniferin + H2O | plant cell wall enzyme, involved in lignin biosynthesis | Picea abies | ? | - |
? | |
feruloyl glucoside + H2O | poor substrate | Picea abies | ferulic acid + glucose | - |
? | |
picein + H2O | reaction at 50% (isozyme II) or 12% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | ? | - |
? | |
salicin + H2O | reaction at 25% (isozyme II) or 2% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | ? | - |
? | |
syringin + H2O | reaction at about 30% the rate of 2-nitrophenyl beta-D-glucoside hydrolysis | Picea abies | D-glucose + sinapyl alcohol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 57000, Picea abies, SDS-PAGE | Picea abies |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Picea abies |
40 | 45 | - |
Picea abies |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 10 (isozyme I), pI: 10.3 (isozyme II) | Picea abies |
4.5 | 5.5 | hypocotyls | Picea abies |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | about half-maximal activity at pH 3.5 and 6.5, hypocotyls | Picea abies |