BRENDA - Enzyme Database show
show all sequences of 3.2.1.126

Characterization of beta-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures

Hösel, W.; Surholt, E.; Borgmann, E.; Eur. J. Biochem. 84, 487-492 (1978)

Data extracted from this reference:

General Stability
General Stability
Organism
0.5 M NaCl stabilizes, removal inactivates
Cicer arietinum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
formononetin 7-beta-glucoside
2-nitrophenyl beta-glucoside
Cicer arietinum
0.4
-
4-methylumbelliferyl beta-D-glucoside
ferulic acid p-beta-glucoside, 4-nitrophenyl beta-D-glucoside
Cicer arietinum
0.6
-
Picein
-
Cicer arietinum
0.8
-
coniferin
-
Cicer arietinum
0.9
-
4-Methylumbelliferyl beta-D-glucosaminide
-
Cicer arietinum
3
-
2-nitrophenyl beta-D-galactoside
-
Cicer arietinum
5
-
syringin
4-nitrophenyl beta-D-glucoside
Cicer arietinum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell wall
-
Cicer arietinum
5618
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
63000
-
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
110000
-
Cicer arietinum, gel filtration
Cicer arietinum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cicer arietinum
-
chickpea
-
Purification (Commentary)
Commentary
Organism
to near homogeneity
Cicer arietinum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Cicer arietinum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7
-
-
Cicer arietinum
Storage Stability
Storage Stability
Organism
4°C, in the presence of 0.5 M NaCl, several months
Cicer arietinum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nitrophenyl beta-D-galactoside + H2O
-
26041
Cicer arietinum
2-nitrophenol + D-galactose
-
-
-
-
2-nitrophenyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
2-nitrophenol + D-glucose
-
-
-
-
4-methylumbelliferyl beta-D-glucosaminide + H2O
poor substrate
26041
Cicer arietinum
4-methylumbelliferone + D-glucosamine
-
-
-
-
4-methylumbelliferyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
4-methylumbelliferone + D-glucose
-
-
-
-
4-nitrophenyl beta-D-galactoside + H2O
-
26041
Cicer arietinum
4-nitrophenol + D-galactose
-
-
-
-
4-nitrophenyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
4-nitrophenol + D-glucose
-
-
-
-
coniferin + H2O
-
26041
Cicer arietinum
coniferol + D-glucose
-
26041
Cicer arietinum
-
ferulic acid p-beta-glucoside + H2O
-
26041
Cicer arietinum
ferulic acid + D-glucose
-
-
-
-
additional information
very poor substrates are arbutin or indican, no substrates are alpha-glucosides, 4-methylumbelliferyl beta-cellobioside, 4-methylumbelliferyl beta-glucuronide, quercetin 3-rhamnosyl glucoside, quercetin 3-rhamnoside or cellobiose
26041
Cicer arietinum
?
-
-
-
-
picein + H2O
-
26041
Cicer arietinum
?
-
-
-
-
salicin + H2O
poor substrate
26041
Cicer arietinum
?
-
-
-
-
syringin + H2O
poor substrate
26041
Cicer arietinum
D-glucose + sinapyl alcohol
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Cicer arietinum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Cicer arietinum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
7.5
about 20% of maximal activity at pH 4 and 7.5
Cicer arietinum
General Stability (protein specific)
General Stability
Organism
0.5 M NaCl stabilizes, removal inactivates
Cicer arietinum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
formononetin 7-beta-glucoside
2-nitrophenyl beta-glucoside
Cicer arietinum
0.4
-
4-methylumbelliferyl beta-D-glucoside
ferulic acid p-beta-glucoside, 4-nitrophenyl beta-D-glucoside
Cicer arietinum
0.6
-
Picein
-
Cicer arietinum
0.8
-
coniferin
-
Cicer arietinum
0.9
-
4-Methylumbelliferyl beta-D-glucosaminide
-
Cicer arietinum
3
-
2-nitrophenyl beta-D-galactoside
-
Cicer arietinum
5
-
syringin
4-nitrophenyl beta-D-glucoside
Cicer arietinum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell wall
-
Cicer arietinum
5618
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
63000
-
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
110000
-
Cicer arietinum, gel filtration
Cicer arietinum
Purification (Commentary) (protein specific)
Commentary
Organism
to near homogeneity
Cicer arietinum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Cicer arietinum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7
-
-
Cicer arietinum
Storage Stability (protein specific)
Storage Stability
Organism
4°C, in the presence of 0.5 M NaCl, several months
Cicer arietinum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nitrophenyl beta-D-galactoside + H2O
-
26041
Cicer arietinum
2-nitrophenol + D-galactose
-
-
-
-
2-nitrophenyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
2-nitrophenol + D-glucose
-
-
-
-
4-methylumbelliferyl beta-D-glucosaminide + H2O
poor substrate
26041
Cicer arietinum
4-methylumbelliferone + D-glucosamine
-
-
-
-
4-methylumbelliferyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
4-methylumbelliferone + D-glucose
-
-
-
-
4-nitrophenyl beta-D-galactoside + H2O
-
26041
Cicer arietinum
4-nitrophenol + D-galactose
-
-
-
-
4-nitrophenyl beta-D-glucoside + H2O
-
26041
Cicer arietinum
4-nitrophenol + D-glucose
-
-
-
-
coniferin + H2O
-
26041
Cicer arietinum
coniferol + D-glucose
-
26041
Cicer arietinum
-
ferulic acid p-beta-glucoside + H2O
-
26041
Cicer arietinum
ferulic acid + D-glucose
-
-
-
-
additional information
very poor substrates are arbutin or indican, no substrates are alpha-glucosides, 4-methylumbelliferyl beta-cellobioside, 4-methylumbelliferyl beta-glucuronide, quercetin 3-rhamnosyl glucoside, quercetin 3-rhamnoside or cellobiose
26041
Cicer arietinum
?
-
-
-
-
picein + H2O
-
26041
Cicer arietinum
?
-
-
-
-
salicin + H2O
poor substrate
26041
Cicer arietinum
?
-
-
-
-
syringin + H2O
poor substrate
26041
Cicer arietinum
D-glucose + sinapyl alcohol
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 43000 + 1 * 63000, Cicer arietinum, SDS-PAGE
Cicer arietinum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Cicer arietinum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Cicer arietinum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
7.5
about 20% of maximal activity at pH 4 and 7.5
Cicer arietinum
Other publictions for EC 3.2.1.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751886
Baiya
Demonstration of monolignol b ...
Oryza sativa Japonica Group
Plant Physiol. Biochem.
127
223-230
2018
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751432
Tsuyama
-
Coniferin beta-glucosidase is ...
Populus sieboldii x Populus grandidentata
J. Wood Sci.
61
438-444
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682290
Escamilla-Trevino
Arabidopsis thaliana beta-gluc ...
Arabidopsis thaliana
Phytochemistry
67
1651-1660
2006
-
-
1
-
-
-
-
3
-
-
-
-
-
6
-
-
1
-
-
4
4
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
4
4
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650284
Watt
Agrobacterium tumefaciens beta ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1385
78-88
1998
6
-
1
-
-
-
5
10
-
-
2
1
-
1
-
-
1
1
-
-
1
-
12
1
1
-
-
11
1
-
-
-
4
1
-
6
-
1
-
-
-
-
-
5
4
10
-
-
2
1
-
-
-
1
-
-
1
-
12
1
1
-
-
11
1
-
-
1
-
-
-
-
-
-
653495
Dharmawardhana
A beta-glucosidase from lodgep ...
Pinus contorta
Plant Physiol.
107
331-339
1995
-
-
-
-
-
-
2
4
-
-
2
1
-
3
-
1
1
-
-
1
1
-
8
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
4
-
-
2
1
-
-
1
1
-
1
1
-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
26044
Leinhos
Purification of an acidic coni ...
Pinus banksiana
Phytochemistry
37
311-315
1994
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
1
1
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
1
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
26042
Hösel
-
Relationship of coniferin beta ...
Petroselinum crispum, Triticum aestivum
Plant Cell Tissue Organ Cult.
1
137-148
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
26041
Hösel
Characterization of beta-gluco ...
Cicer arietinum
Eur. J. Biochem.
84
487-492
1978
-
-
-
-
-
1
-
7
1
-
3
-
-
1
-
-
1
-
-
1
1
1
12
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
7
1
-
3
-
-
-
-
1
-
1
1
1
12
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
26043
Marcinowski
Enzymology of lignification. C ...
Picea abies
Eur. J. Biochem.
87
37-44
1978
-
-
-
-
-
-
-
7
1
-
2
1
-
1
-
-
1
-
-
3
1
2
13
1
2
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
1
-
2
1
-
-
-
1
-
3
1
2
13
1
2
-
-
-
2
1
-
-
-
-
-
-
-
-