Cloned (Comment) | Organism |
---|---|
for heterologous expression, RG cDNA cloned into the pSE280 vector. For optimum expression, RG cDNA cloned into the pQE-2 vector and expressed in Escherichia coli strain M15 | Rauvolfia serpentina |
Crystallization (Comment) | Organism |
---|---|
by the hanging-drop vapour diffusion technique at 20°C. Crystals reach a maximum dimension of about 0.2 * 0.15 * 0.05 mm, belong to space group I222 and diffract to 2.30 A resolution with unit-cell parameters of a = 102.8 A, b = 127.3 A and c = 215.8 A | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Raucaffricine | - |
Rauvolfia serpentina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60930 | - |
sequence analysis | Rauvolfia serpentina |
61000 | - |
isolated from cell suspension | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1200fold, by Ni-NTA column | Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroraucaffricine + H2O | - |
Rauvolfia serpentina | 2beta(R)-1,2-dihydrovomilenine + D-glucose | - |
? | |
raucaffricine + H2O | best substrate | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
strictosidine + H2O | - |
Rauvolfia serpentina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Rauvolfia serpentina |
monomer | in solution | Rauvolfia serpentina |
Synonyms | Comment | Organism |
---|---|---|
raucaffricine glucosidase | - |
Rauvolfia serpentina |