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Literature summary for 3.2.1.125 extracted from
- Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis (2000), Phytochemistry, 54, 657-666.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the RG product vomilenine is a direct intermediate in ajmaline biosynthesis and utilization of raucaffricine for the ajmaline biosynthestic pathway could be a crucial and rate-limiting step in the formation of the antiarrythmic drug ajmaline | Rauvolfia serpentina |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli TOP 10, sequencing | Rauvolfia serpentina |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
Raucaffricine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina |  |
| 1.8 | - |
strictosidine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60931 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
| 61000 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raucaffricine + H2O | Rauvolfia serpentina | specifically involved in alkaloid biosynthesis, e.g. ajmaline | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | Q9SPP9 | BENTH. ex KURZ | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant RG | Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Rauvolfia serpentina | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
| 1-methyl-1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
| 21-glucopyranosyl-hydroxysarpagan-17-al + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
| 21-glucopyranosyl-hydroxysarpagan-17-ol + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
| 5alpha-carboxystrictosidine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
| raucaffricine + H2O | best substrate, highly specific enzyme | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
| raucaffricine + H2O | specifically involved in alkaloid biosynthesis, e.g. ajmaline | Rauvolfia serpentina | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? | |
| strictosidine + H2O | only recombinant RG, no substrate of native RG | Rauvolfia serpentina | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| additional information | belongs to family1 of glycosyl hydrolases | Rauvolfia serpentina |
| RG | - |
Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Rauvolfia serpentina |
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