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Literature summary for 3.2.1.125 extracted from
- Purification, partial amino acid sequence and structure of the product of raucaffricine-O-beta-D-glucosidase from plant cell cultures of Rauwolfia serpentina (1999), Phytochemistry, 50, 1099-1109.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Rauvolfia serpentina | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, SDS-PAGE | Rauvolfia serpentina |
| 63000 | 66000 | gel filtration | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raucaffricine + H2O | Rauvolfia serpentina | involved in the biosynthesis of alkaloids, e.g. ajmaline | D-glucose + vomilenine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Rauvolfia serpentina |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1170fold | Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Rauvolfia serpentina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.1 | - |
pH 5, 30°C | Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raucaffricine + H2O | - |
Rauvolfia serpentina | D-glucose + vomilenine | the product vomilenine exists in aqueous solutions in an equilibrium of 21(R)- and 21(S)-vomilenine in a ratio of 3.4:1 | ? | |
| raucaffricine + H2O | involved in the biosynthesis of alkaloids, e.g. ajmaline | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Rauvolfia serpentina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| additional information | member of the family1 of glycosyl hydrolases | Rauvolfia serpentina |
| RG | - |
Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Rauvolfia serpentina |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rauvolfia serpentina | isoelectric focusing | - |
5.8 |
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