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Literature summary for 3.2.1.125 extracted from

  • Schuebel, H.; Stoeckigt, J.; Feicht, R.; Simon, H.
    Partial purification and characterization of raucaffricine beta-D-glucosidase from plant cell-suspension cultures of Rauwolfia serpentina Benth (1986), Helv. Chim. Acta, 69, 538-547.
No PubMed abstract available
Search for more literature for 3.2.1.125

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 0.95 M: 30% inhibition Rauvolfia serpentina
D-glucose 0.8 M: complete inhibition Rauvolfia serpentina
additional information D-glucono-1,5-lactone up to 1 M, phenylmethylsulfonyl fluoride up to 80 mM, EDTA up to 80 mM, iodoacetamide up to 80 mM, strictosidine or amygdalin up to 50 mM, mannitol up to 1 M are no inhibitors Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 Raucaffricine
-
 Rauvolfia serpentina
1.4
-
 1,2-Dihydro-1-methylraucaffricine
-
 Rauvolfia serpentina
1.5
-
 1,2-Dihydroraucaffricine
-
 Rauvolfia serpentina
1.7
-
 17-O-Deacetyl-1,2-dihydroraucaffricine
-
 Rauvolfia serpentina
2
-
 1,2,19,20-Tetrahydroraucaffricine
-
 Rauvolfia serpentina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66600
-
 gel filtration Rauvolfia serpentina

Organism

Organism UniProt Comment Textmining
Rauvolfia caffra
-
-
-
Rauvolfia mannii
-
-
-
Rauvolfia serpentina
-
-
-
Rauvolfia verticillata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rauvolfia serpentina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.5
-
 reaction with raucaffricine Rauvolfia serpentina

Storage Stability

Storage Stability Organism
4°C, crude enzyme, 30% loss of activity within 6 months Rauvolfia serpentina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,19,20-tetrahydroraucaffricine + H2O 87% of activity compared to raucaffricine Rauvolfia serpentina 17-O-acetylnorajmaline + D-glucose
-
 ?
1,2-dihydro-1-methylraucaffricine + H2O 36% of activity compared to raucaffricine Rauvolfia serpentina acetic acid 3-ethylidene-4-hydroxy-13-methyl-1,3,4,7,8,13,13a,13b-octahydro-2H,6H-2,7-cyclo-6,8a-methano-pyrido[1',2':1,2]azepino[3,4-b]indol-8-yl ester + D-glucose
-
 ?
1,2-dihydroraucaffricine + H2O 98% of activity compared to raucaffricine Rauvolfia serpentina 2beta(R)-1,2-dihydrovomilenine + D-glucose
-
 ?
17-O-deacetyl-1,2-dihydroraucaffricine + H2O 95% of activity compared to raucaffricine Rauvolfia serpentina 3-ethylidene-1,3,4,7,8,13,13a,13b-octahydro-2H,6H-2,7-cyclo-6,8a-methano-pyrido[1',2':1,2]azepino[3,4-b]indole-4,8-diol + D-glucose
-
 ?
raucaffricine + H2O
-
 Rauvolfia serpentina vomilenine + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Rauvolfia serpentina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 20°C: about 55% of maximal activity, 55°C: about 40% of maximal activity Rauvolfia serpentina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, 50% loss of activity Rauvolfia serpentina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
 Rauvolfia serpentina

pH Range

pH Minimum pH Maximum Comment Organism
4.2 6 half-maximal activity at pH 4.2 and pH 6.0 Rauvolfia serpentina

pI Value

Organism Comment pI Value Maximum pI Value
Rauvolfia serpentina isoelectric focusing
-
 5.8