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Literature summary for 3.2.1.125 extracted from

  • Xia, L.; Lin, H.; Staniek, A.; Panjikar, S.; Ruppert, M.; Hilgers, P.; Williardt, J.; Rajendran, C.; Wang, M.; Warzecha, H.; Jäger, V.; Stöckigt, J.
    Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours (2015), J. Enzyme Inhib. Med. Chem., 30, 472-478.
    View publication on PubMed
Search for more literature for 3.2.1.125

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Rauvolfia serpentina

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with the (1R,2S,3S,4R,5R)-4-(cyclohexylmethylamino)-5-(hydroxymethyl)cyclopentane-1,2,3-triol Rauvolfia serpentina

Inhibitors

Inhibitors Comment Organism Structure
N-(bromobenzyl)-beta-D-gluco-1,5-deoxa-pyranosylamine the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates Rauvolfia serpentina
N-(cyclohexyl)-beta-D-gluco-1,5-deoxa-pyranosylamine the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates Rauvolfia serpentina
N-(cyclohexylmethyl)-beta-D-gluco-1,5-deoxa-pyranosylamine the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
raucaffricine + H2O Rauvolfia serpentina
-
 D-glucose + vomilenine
-
 ?

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina Q9SPP9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raucaffricine + H2O
-
 Rauvolfia serpentina D-glucose + vomilenine
-
 ?

Synonyms

Synonyms Comment Organism
raucaffricine glucosidase
-
 Rauvolfia serpentina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rauvolfia serpentina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rauvolfia serpentina

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000066
-
 N-(bromobenzyl)-beta-D-gluco-1,5-deoxa-pyranosylamine 30°C, pH 5.0 Rauvolfia serpentina
0.00022
-
 N-(cyclohexylmethyl)-beta-D-gluco-1,5-deoxa-pyranosylamine 30°C, pH 5.0 Rauvolfia serpentina
0.628
-
 N-(cyclohexyl)-beta-D-gluco-1,5-deoxa-pyranosylamine 30°C, pH 5.0 Rauvolfia serpentina