Literature summary for 3.2.1.123 extracted from

Ishibashi, Y.; Nakasone, T.; Kiyohara, M.; Horibata, Y.; Sakaguchi, K.; Hijikata, A.; Ichinose, S.; Omori, A.; Yasui, Y.; Imamura, A.; Ishida, H.; Kiso, M.; Okino, N.; Ito, M.
A novel endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides (2007), J. Biol. Chem., 282, 11386-11396.

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Cloned(Commentary)

Cloned (Comment)	Organism
amplified gene product encoding amino acid residues 23-488 without the signal peptide digested with HindIII and NotI and inserted into the corresponding sites of pET22b(+) to generate a C-terminal His6-tagged protein. The recombinant plasmid and mutants expressed in Escherichia coli strain BL21(DE3)	Rhodococcus equi

Protein Variants

Protein Variants	Comment	Organism
E234D	strongly decreased enzyme activity compared with the wild-type enzyme	Rhodococcus equi
E234Q	no activity	Rhodococcus equi
E341D	strongly decreased enzyme activity compared with the wild-type enzyme	Rhodococcus equi
E341Q	no activity	Rhodococcus equi

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Rhodococcus equi
Fe3+	-	Rhodococcus equi
Hg2+	completely inhibits enzyme activity	Rhodococcus equi
additional information	Ca2+, Mg2+, Mn2+, Ni2+, Co2+, and EDTA have no significant effect on the activity	Rhodococcus equi
taurodeoxycholate	strongly inhibits the enzyme activity at a concentration of 0.05%	Rhodococcus equi
Zn2+	-	Rhodococcus equi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	Galbeta1-6Galbeta1-6Galbeta1-1'ceramide	-	Rhodococcus equi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52299	-	x * 54000, SDS-PAGE, x * 52299, sequence analysis	Rhodococcus equi
54000	-	x * 54000, SDS-PAGE, x * 52299, sequence analysis	Rhodococcus equi

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus equi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration, 2100fold	Rhodococcus equi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Galalpha1-6Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O	most rapidly hydrolyzed	Rhodococcus equi	ceramide + Galalpha1-6Galalpha1-6Gal	-	?
Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O	-	Rhodococcus equi	ceramide + Galalpha1-6Galbeta1-6Gal	-	?
Galbeta1-6Galbeta1-1'ceramide + H2O	-	Rhodococcus equi	ceramide + Galbeta1-6Galbeta	-	?
Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O	-	Rhodococcus equi	ceramide + Galbeta1-6Galbeta1-6Gal	-	?
monogalactosylceramide + H2O	-	Rhodococcus equi	ceramide + D-galactose	-	?
additional information	does not hydrolyze ganglio-, globo-, or lacto-series glycosphingolipids	Rhodococcus equi	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 54000, SDS-PAGE, x * 52299, sequence analysis	Rhodococcus equi

Synonyms

Synonyms	Comment	Organism
ceramide glycanase	-	Rhodococcus equi
EGALC	-	Rhodococcus equi
endogalactosylceramidase	-	Rhodococcus equi
endoglycoceramidase	-	Rhodococcus equi
oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase	-	Rhodococcus equi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13.3	-	Galbeta1-6Galbeta1-6Galbeta1-1'ceramide	-	Rhodococcus equi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Rhodococcus equi

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rhodococcus equi	sequence analysis	-	4.28

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Release 2023.1 (January 2023)