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Literature summary for 3.2.1.123 extracted from

  • Ito, M.; Yamagata, T.
    Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities (1989), J. Biol. Chem., 264, 9510-9519.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
Nonidet P-40 activates Rhodococcus sp.
sodium cholate
-
 Rhodococcus sp.
sodium taurodeoxycholate activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v Rhodococcus sp.
Triton X-100 activates Rhodococcus sp.
Triton X-100 activates endoglyceroceramidase II at 0.2-0.4% Rhodococcus sp.
Triton X-100 activates endoglyceroceramidase I at 0.3-0.4% Rhodococcus sp.

General Stability

General Stability Organism
unstable at protein concentration below 0.2 mg/ml or without Triton X-100 Rhodococcus sp.
unstable to repeated freezing and thawing Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 63% inhibition of endoglycoceramidase I, 94% inhibition of endoglycoceramidase II Rhodococcus sp.
Brij 58
-
 Rhodococcus sp.
Cu2+
-
 Rhodococcus sp.
Hg2+
-
 Rhodococcus sp.
Tween 20
-
 Rhodococcus sp.
Zn2+ 1 mM, 20% inhibition of endoglycoceramidase I, 48% inhibition of endoglycoceramidase II Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase I Rhodococcus sp.
0.21
-
 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of sodium taurodeoxycholate, endoglycoceramidase II Rhodococcus sp.
0.23
-
 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase I Rhodococcus sp.
0.5
-
 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide pH 5, 37°C, in presence of Triton X-100, endoglycoceramidase II Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32700
-
 endoglycoceramidase I, gel filtration Rhodococcus sp.
43000
-
 endoglycoceramidase II, gel filtration Rhodococcus sp.
55900
-
 endoglycoceramidase I, PAGE in absence and presence of SDS Rhodococcus sp.
58000
-
 1 * 58000, SDS-PAGE, endoglyceroceramidase II Rhodococcus sp.
58900
-
 endoglycoceramidase II, PAGE in absence and presence of SDS Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 2 molecular species: I
-
Rhodococcus sp.
-
 molecular species: II
-
Rhodococcus sp.
-
 mutant strain M750
-

Purification (Commentary)

Purification (Comment) Organism
2 molecular species: EGCaseI and EGCaseII, homogeneity Rhodococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.5
-
 isoform I Rhodococcus sp.

Storage Stability

Storage Stability Organism
0°C, protein concentration greater than 0.2 mg/ml, 50 mM sodium acetate buffer at pH 6.0 or 20 mM phosphate buffer at pH 7.2, 0.1-1.0% Triton X-100, stable for several months Rhodococcus sp.
frozen, stable for at least 6 months Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb3Cer Rhodococcus sp. Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Rhodococcus sp. Galbeta(1-4)Glc + ceramide
-
 ?
GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb5Cer Rhodococcus sp. GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gb4Cer Rhodococcus sp. GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Rhodococcus sp. GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
glycosphingolipid + H2O strongly resistant to hydrolysis by endoglycoceramidase II in comparison with endoglycoceramidase I Rhodococcus sp. ?
-
 ?
glycosphingolipid + H2O ganglio-type Rhodococcus sp. ?
-
 ?
glycosphingolipid + H2O neutral and acidic in presence of 0.1% Triton X-100, lacto-type Rhodococcus sp. ?
-
 ?
additional information no substrate: sulfatides Rhodococcus sp. ?
-
 ?
additional information no substrate: sphingomyelins Rhodococcus sp. ?
-
 ?
additional information no substrate: gala-type glycosphingolipids Rhodococcus sp. ?
-
 ?
additional information no substrate: cerebrosides Rhodococcus sp. ?
-
 ?
additional information no substrate: glycoglycerolipids Rhodococcus sp. ?
-
 ?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55900, SDS-PAGE, endoglyceroceramidase I Rhodococcus sp.
monomer 1 * 58000, SDS-PAGE, endoglyceroceramidase II Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 60 min, pH 3, 50.4% of endoglycoceramidase I activity and 27.5% of endoglycoceramidase II activity lost Rhodococcus sp.
37
-
 60 min pH 4-8, endoglycoceramidase I and II, stable Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 endoglycoceramidase I Rhodococcus sp.
5.5
-
 endoglycoceramidase II Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable for 60 min Rhodococcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus sp. endoglycoceramidase I
-
 3.5
Rhodococcus sp. endoglycoceramidase II
-
 4.5