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Literature summary for 3.2.1.123 extracted from

  • Ashida, H.; Yamamoto, K.; Kumagai, H.; Tochikura, T.
    Purification and characterization of membrane-bound endoglycoceramidase from Corynebacterium sp (1992), Eur. J. Biochem., 205, 729-735.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
Nonidet P-40 activates at 0.2% Corynebacterium sp.
Triton X-100 activates at 0.2% Corynebacterium sp.

General Stability

General Stability Organism
stable to repeated freezing and thawing Corynebacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 85% inhibition at 1 mM Corynebacterium sp.
Zn2+ 50% inhibition at 1 mM Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide pH 6.5, 37°C Corynebacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane bound Corynebacterium sp.
-
-
extracellular
-
 Corynebacterium sp.
-
-
intracellular
-
 Corynebacterium sp. 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Corynebacterium sp.
250000 450000 gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Corynebacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Corynebacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
227.6
-
-
 Corynebacterium sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least 1 year Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Corynebacterium sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
glycosphingolipid + H2O glycosphingolipids readily hydrolyzed, globo-type (slowly) Corynebacterium sp. ?
-
 ?
glycosphingolipid + H2O ganglio-type Corynebacterium sp. ?
-
 ?
glycosphingolipid + H2O neutral and acidic in presence of 0.1% Triton X-100, lacto-type Corynebacterium sp. ?
-
 ?
glycosphingolipid + H2O glycosphingolipids, overview Corynebacterium sp. ?
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
 Corynebacterium sp. NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 7.0, 10 min, stable up to Corynebacterium sp.
60
-
 pH 7.0, 10 min, complete inactivation Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
 Corynebacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 stable Corynebacterium sp.
4.5 8 37°C, 30 min, stable Corynebacterium sp.