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Literature summary for 3.2.1.123 extracted from

  • Ito, M.; Yamagata, T.
    A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids [published erratum appears in J Biol Chem 1987 Feb 5;262(4):1926] (1986), J. Biol. Chem., 261, 14278-14282.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
taurodeoxycholate 0.5 mg/ml, 2.5fold increase of activity Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Rhodococcus sp.
Hg2+
-
 Rhodococcus sp.
Zn2+
-
 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 G-74-2
-
Rhodococcus sp. G-74-2
-
 G-74-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glcbeta(1-1)ceramide + H2O 84% of the activity with galactosylcerebroside Rhodococcus sp. Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. G-74-2 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. G-74-2 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Rhodococcus sp. Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-6)Galbeta(1-6)Galbeta(1-1)ceramide + H2O i.e. neogalactosylceramide, 77% of the activity with lactosylceramide Rhodococcus sp. Galbeta(1-6)Galbeta(1-6)Gal + ceramide
-
 ?
GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GM2, 94% of the activity with lactosylceramide Rhodococcus sp. GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 35% of the activity with lactosylceramide Rhodococcus sp. GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glc + ceramide
-
 ?
Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 88% of the activity with lactosylceramide Rhodococcus sp. Manalpha(1-3)Manbeta(1-4)Glc + ceramide
-
 ?
Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 93% of the activity with lactosylceramide Rhodococcus sp. Manbeta(1-4)Glc + ceramide
-
 ?
additional information no substrate: N-linked glycoproteins Rhodococcus sp. ?
-
 ?
additional information no substrate: O-linked glycoproteins Rhodococcus sp. ?
-
 ?
additional information hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids Rhodococcus sp. ?
-
 ?
additional information no substrate: N-linked glycoproteins Rhodococcus sp. G-74-2 ?
-
 ?
additional information no substrate: O-linked glycoproteins Rhodococcus sp. G-74-2 ?
-
 ?
additional information hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids Rhodococcus sp. G-74-2 ?
-
 ?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD, 93% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT1b Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O 93% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. sialylparagloboside, as active as lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT, 81% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable Rhodococcus sp.