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Literature summary for 3.2.1.122 extracted from
- Metabolism of sucrose and its five isomers by Fusobacterium mortiferum (2002), Microbiology, 148, 843-852.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | MalH is induced during growth on the sucrose isomers trehalulose, turanose, maltulose, leucrose and palatinose, but not on sucrose | Fusobacterium mortiferum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| structural organization of the putative alpha-glycoside operon | Klebsiella pneumoniae |
| structural organization of the putative alpha-glycoside operon | Escherichia coli |
| structural organization of the putative alpha-glycoside operon, gene malH is adjacent to malB and malR, malH gene is cloned and expressed in Escherichia coli PEP43 | Fusobacterium mortiferum |
General Stability
| General Stability | Organism |
|---|---|
| unstable enzyme | Fusobacterium mortiferum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Klebsiella pneumoniae |  |
| Co2+ | activates | Fusobacterium mortiferum | |
| Mn2+ | - |
Klebsiella pneumoniae | |
| Mn2+ | cofactor, requirement | Fusobacterium mortiferum | |
| additional information | metal-dependent | Fusobacterium mortiferum | |
| additional information | metal-dependent | Klebsiella pneumoniae | |
| additional information | requires a divalent metal ion, e.g. Mn2+ | Fusobacterium mortiferum | |
| Ni2+ | - |
Klebsiella pneumoniae | |
| Ni2+ | activates | Fusobacterium mortiferum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49256 | - |
x * 49256, calculated from the amino acid sequence | Klebsiella pneumoniae |
| 49386 | - |
x * 49386, calculated from the amino acid sequence | Escherichia coli |
| 49712 | - |
4 * 49712, calculated from the amino acid sequence | Fusobacterium mortiferum |
| 50000 | - |
4 * 50000, SDS-PAGE | Fusobacterium mortiferum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Klebsiella pneumoniae | - |
? | - |
? | |
| additional information | Fusobacterium mortiferum | carbohydrate metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q8XEI0 | enterohaemorrhagic strain O157:H7, GlvG | - |
| Fusobacterium mortiferum | O06901 | strain ATCC 25557, MalH | - |
| Klebsiella pneumoniae | Q9AGA6 | strain ATCC 23357, AglB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant MalH expressed in Escherichia coli PEP43 | Fusobacterium mortiferum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Fusobacterium mortiferum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Fusobacterium mortiferum | alpha-D-glucose 6-phosphate + 4-methylumbelliferone | fluorescent product | ? | |
| leucrose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
| leucrose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
| maltulose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
| maltulose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
| additional information | - |
Klebsiella pneumoniae | ? | - |
? | |
| additional information | not: cellobiose 6-phosphate, gentiobiose 6-phosphate | Fusobacterium mortiferum | ? | - |
? | |
| additional information | not: D-glucose, D-fructose, non-phosphorylated sucrose isomers | Fusobacterium mortiferum | ? | - |
? | |
| additional information | substrate specificity, not: sucrose 6-phosphate | Fusobacterium mortiferum | ? | - |
? | |
| additional information | substrate specificity, not: sucrose 6-phosphate | Klebsiella pneumoniae | ? | - |
? | |
| additional information | carbohydrate metabolism | Fusobacterium mortiferum | ? | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Fusobacterium mortiferum | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
| p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
| p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O | - |
Fusobacterium mortiferum | beta-D-glucose 6-phosphate + p-nitrophenol | - |
? | |
| palatinose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
| palatinose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
| trehalulose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | alpha-D-glucose 6-phosphate + D-fructose | - |
? | |
| trehalulose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucose 6-phosphate + D-fructose | - |
? | |
| turanose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
| turanose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Fusobacterium mortiferum |
| ? | x * 49256, calculated from the amino acid sequence | Klebsiella pneumoniae |
| ? | x * 49386, calculated from the amino acid sequence | Escherichia coli |
| tetramer | 4 * 50000, SDS-PAGE | Fusobacterium mortiferum |
| tetramer | 4 * 49712, calculated from the amino acid sequence | Fusobacterium mortiferum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AglB | - |
Klebsiella pneumoniae |
| GlvG | - |
Escherichia coli |
| MalH | - |
Fusobacterium mortiferum |
| More | belongs to familiy 4 of the glycosylhydrolase superfamily | Fusobacterium mortiferum |
| More | belongs to familiy 4 of the glycosylhydrolase superfamily | Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Fusobacterium mortiferum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Fusobacterium mortiferum |
| 7.5 | - |
assay at | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | requires a divalent metal ion, e.g. Mn2+ | Fusobacterium mortiferum | |
| NAD+ | - |
Klebsiella pneumoniae | |
| NAD+ | cofactor, requirement | Fusobacterium mortiferum | |
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