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Literature summary for 3.2.1.122 extracted from

  • Thompson, J.; Gentry-Weeks, C.R.; Nguyen, N.Y.; Folk, J.E.; Robrish, S.A.
    Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides (1995), J. Bacteriol., 177, 2505-2512.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
additional information growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis Fusobacterium mortiferum

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli Fusobacterium mortiferum

General Stability

General Stability Organism
dialysis and storage overnight results in 90% loss of activity Fusobacterium mortiferum
freezing and thawing from -20°C retains 50% of initial activity Fusobacterium mortiferum
inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification Fusobacterium mortiferum
purification by Rotofor isoelectric focusing inactivates Fusobacterium mortiferum

Inhibitors

Inhibitors Comment Organism Structure
air exposure to air rapidly inactivates Fusobacterium mortiferum
sulfhydryl-reactive agent rapidly inactivates Fusobacterium mortiferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 maltose 6'-phosphate pH 7, 37°C Fusobacterium mortiferum
2
-
 p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate pH 7, 37°C Fusobacterium mortiferum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Fusobacterium mortiferum
Fe2+ activates Fusobacterium mortiferum
Mn2+ activates Fusobacterium mortiferum
additional information not activated by Sr2+ Fusobacterium mortiferum
additional information metal-dependent Fusobacterium mortiferum
additional information not activated by Mg2+, Zn2+ or Ca2+ Fusobacterium mortiferum
Ni2+ activates Fusobacterium mortiferum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE Fusobacterium mortiferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose 6'-phosphate + H2O Fusobacterium mortiferum in the pathway of maltose fermentation D-glucose 6-phosphate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Fusobacterium mortiferum
-
 strain ATCC 25557
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive enzyme, rapidly inactivated by exposure to air Fusobacterium mortiferum

Purification (Commentary)

Purification (Comment) Organism
6fold, dithiothreitol and Mn2+ is required for stabilization during purification Fusobacterium mortiferum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.67
-
 at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis Fusobacterium mortiferum

Storage Stability

Storage Stability Organism
-20°C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable Fusobacterium mortiferum
storage overnight, 90% loss of activity Fusobacterium mortiferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose 6'-phosphate + H2O broad substrate specificity Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose molar ratio is 1:1 ?
maltose 6'-phosphate + H2O in the pathway of maltose fermentation Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose
-
 ?
additional information not: nonphosphorylated alpha- and beta-glycosides, p-nitrophenyl-beta-D-glucopyranoside 6-phosphate, p-nitrophenyl-alpha-D-mannopyranoside 6-phosphate, very poor substrate: p-nitrophenyl-alpha-D-galactopyranoside 6-phosphate Fusobacterium mortiferum ?
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum alpha-D-glucose 6-phosphate + p-nitrophenol molar ratio is 1:1 ?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum alpha-D-glucose 6-phosphate + p-nitrophenol yellow product ?
palatinose 6'-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-fructose
-
 ?
sucrose 6-phosphate + H2O hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio Fusobacterium mortiferum D-glucose 6-phosphate + D-fructose
-
 ?
trehalose 6-phosphate + H2O hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio Fusobacterium mortiferum ?
-
 ?
turanose 6'-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-fructose
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Fusobacterium mortiferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Fusobacterium mortiferum
40
-
 maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis Fusobacterium mortiferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis Fusobacterium mortiferum
7
-
 assay at Fusobacterium mortiferum

pI Value

Organism Comment pI Value Maximum pI Value
Fusobacterium mortiferum analytical isoelectric focusing
-
 4.9