Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.79 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
2.35 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
4.03 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligoxyloglucan + H2O | Aspergillus oryzae | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | isoprimeverose + ? | - |
? | |
oligoxyloglucan + H2O | Aspergillus oryzae ATCC 42149 | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | isoprimeverose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q2U8V9 | - |
- |
Aspergillus oryzae ATCC 42149 | Q2U8V9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 4.5, 60°C | Aspergillus oryzae |
1.32 | - |
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C | Aspergillus oryzae |
226 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
296 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
297 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
422 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
427 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glcol, pH 4.5, 60°C | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc + H2O | - |
Aspergillus oryzae ATCC 42149 | ? | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc | - |
? | |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
oligoxyloglucan + H2O | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
oligoxyloglucan + H2O | the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
oligoxyloglucan + H2O | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? | |
oligoxyloglucan + H2O | the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPase | - |
Aspergillus oryzae |
IpeA | - |
Aspergillus oryzae |
isoprimeverose-producing oligoxyloglucan hydrolase | - |
Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
532 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
661 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
1216 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae |
Organism | Comment | Expression |
---|---|---|
Aspergillus oryzae | the enzyme is expressed in xylose and xyloglucan media and is strongly induced in the presence of xyloglucan endo-xyloglucanase-hydrolyzed products | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
132 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
282 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
678 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae |