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Literature summary for 3.2.1.119 extracted from

  • Lizotte, P.A.; Poulton, J.E.
    Catabolism of cyanogenic glycosides by purified vicianin hydrolase from Squirrel's foot fern (Davallia trichomanoides Blume) (1988), Plant Physiol., 86, 322-324.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.119

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
castanospermine indolizidine alkaloid, strong, with (R)-prunazin as substrate Davallia trichomanoides
Cu2+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
delta-gluconolactone strong, with (R)-prunazin as substrate Davallia trichomanoides
diethyldithiocarbamate weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
Fe2+ weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
Hg2+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
additional information less than 10% inhibition by Ca2+, Zn2+, Fe3+, Co2+, Cd2+ and Pb2+, no inhibition by EDTA, 2,2'-dipyridyl, o-pheanthroline, 2-mercaptoethanol, dithiothreitol, iodacetic acid, iodoacetamide and N-methylmaleimide Davallia trichomanoides
p-chloromercuriphenylsulfonate strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
 (R)-Prunasin 30°C, pH 6 Davallia trichomanoides
3.39
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6 Davallia trichomanoides
4.9
-
 (R)-Vicianin 30°C, pH 6 Davallia trichomanoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 gel filtration Davallia trichomanoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-vicianin + H2O Davallia trichomanoides involved in catabolism of cyanogenic glycosides mandelonitrile + vicianose
-
 ?

Organism

Organism UniProt Comment Textmining
Davallia trichomanoides
-
-
-
Davallia trichomanoides
-
 Squirrel's foot fern, var. Blume
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Davallia trichomanoides

Purification (Commentary)

Purification (Comment) Organism
-
 Davallia trichomanoides

Source Tissue

Source Tissue Comment Organism Textmining
fiddlehead
-
 Davallia trichomanoides
-
frond young Davallia trichomanoides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-amygdalin + H2O reaction 27% of the rate of vicianine hydrolysis Davallia trichomanoides mandelonitrile + beta-gentiobiose
-
 ?
(R)-amygdalin + H2O
-
 Davallia trichomanoides (R)-mandelonitrile + beta-gentiobiose
-
 ?
(R)-prunasin + H2O reaction 14% of the rate of vicianine hydrolysis Davallia trichomanoides mandelonitrile + D-glucose
-
 ?
(R)-vicianin + H2O i.e. beta-vicianoside of (R)-mandelonitrile, strict requirement for (R)-epimers Davallia trichomanoides mandelonitrile + vicianose
-
 ?
(R)-vicianin + H2O cyanogenic disaccharides preferred over monosaccharides Davallia trichomanoides mandelonitrile + vicianose
-
 ?
(R)-vicianin + H2O involved in catabolism of cyanogenic glycosides Davallia trichomanoides mandelonitrile + vicianose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O poor substrate Davallia trichomanoides 4-nitrophenol + D-glucopyranose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Davallia trichomanoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
5.5
-
 with (R)-vicianin as substrate Davallia trichomanoides
6
-
 with (R)-prunazin as substrate Davallia trichomanoides

pI Value

Organism Comment pI Value Maximum pI Value
Davallia trichomanoides
-
 4.7 4.6