BRENDA - Enzyme Database
show all sequences of 3.2.1.119

Catabolism of cyanogenic glycosides by purified vicianin hydrolase from Squirrel's foot fern (Davallia trichomanoides Blume)

Lizotte, P.A.; Poulton, J.E.; Plant Physiol. 86, 322-324 (1988) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
castanospermine
indolizidine alkaloid, strong, with (R)-prunazin as substrate
Davallia trichomanoides
Cu2+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
delta-gluconolactone
strong, with (R)-prunazin as substrate
Davallia trichomanoides
diethyldithiocarbamate
weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
Fe2+
weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
Hg2+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
additional information
less than 10% inhibition by Ca2+, Zn2+, Fe3+, Co2+, Cd2+ and Pb2+, no inhibition by EDTA, 2,2'-dipyridyl, o-pheanthroline, 2-mercaptoethanol, dithiothreitol, iodacetic acid, iodoacetamide and N-methylmaleimide
Davallia trichomanoides
p-chloromercuriphenylsulfonate
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2
-
(R)-Prunasin
30°C, pH 6
Davallia trichomanoides
3.39
-
p-nitrophenyl-beta-D-glucopyranoside
30°C, pH 6
Davallia trichomanoides
4.9
-
(R)-Vicianin
30°C, pH 6
Davallia trichomanoides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
gel filtration
Davallia trichomanoides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-vicianin + H2O
Davallia trichomanoides
involved in catabolism of cyanogenic glycosides
mandelonitrile + vicianose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Davallia trichomanoides
-
-
-
Davallia trichomanoides
-
Squirrel's foot fern, var. Blume
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Davallia trichomanoides
Purification (Commentary)
Purification (Commentary)
Organism
-
Davallia trichomanoides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fiddlehead
-
Davallia trichomanoides
-
frond
young
Davallia trichomanoides
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-amygdalin + H2O
reaction 27% of the rate of vicianine hydrolysis
646668
Davallia trichomanoides
mandelonitrile + beta-gentiobiose
-
-
-
?
(R)-amygdalin + H2O
-
646668
Davallia trichomanoides
(R)-mandelonitrile + beta-gentiobiose
-
-
-
?
(R)-prunasin + H2O
reaction 14% of the rate of vicianine hydrolysis
646668
Davallia trichomanoides
mandelonitrile + D-glucose
-
-
-
?
(R)-vicianin + H2O
i.e. beta-vicianoside of (R)-mandelonitrile, strict requirement for (R)-epimers
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
(R)-vicianin + H2O
cyanogenic disaccharides preferred over monosaccharides
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
(R)-vicianin + H2O
involved in catabolism of cyanogenic glycosides
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
poor substrate
646668
Davallia trichomanoides
4-nitrophenol + D-glucopyranose
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Davallia trichomanoides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
5.5
-
with (R)-vicianin as substrate
Davallia trichomanoides
6
-
with (R)-prunazin as substrate
Davallia trichomanoides
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Davallia trichomanoides
-
4.7
4.6
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
castanospermine
indolizidine alkaloid, strong, with (R)-prunazin as substrate
Davallia trichomanoides
Cu2+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
delta-gluconolactone
strong, with (R)-prunazin as substrate
Davallia trichomanoides
diethyldithiocarbamate
weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
Fe2+
weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
Hg2+
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
additional information
less than 10% inhibition by Ca2+, Zn2+, Fe3+, Co2+, Cd2+ and Pb2+, no inhibition by EDTA, 2,2'-dipyridyl, o-pheanthroline, 2-mercaptoethanol, dithiothreitol, iodacetic acid, iodoacetamide and N-methylmaleimide
Davallia trichomanoides
p-chloromercuriphenylsulfonate
strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2
-
(R)-Prunasin
30°C, pH 6
Davallia trichomanoides
3.39
-
p-nitrophenyl-beta-D-glucopyranoside
30°C, pH 6
Davallia trichomanoides
4.9
-
(R)-Vicianin
30°C, pH 6
Davallia trichomanoides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
gel filtration
Davallia trichomanoides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-vicianin + H2O
Davallia trichomanoides
involved in catabolism of cyanogenic glycosides
mandelonitrile + vicianose
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Davallia trichomanoides
Purification (Commentary) (protein specific)
Commentary
Organism
-
Davallia trichomanoides
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fiddlehead
-
Davallia trichomanoides
-
frond
young
Davallia trichomanoides
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-amygdalin + H2O
reaction 27% of the rate of vicianine hydrolysis
646668
Davallia trichomanoides
mandelonitrile + beta-gentiobiose
-
-
-
?
(R)-amygdalin + H2O
-
646668
Davallia trichomanoides
(R)-mandelonitrile + beta-gentiobiose
-
-
-
?
(R)-prunasin + H2O
reaction 14% of the rate of vicianine hydrolysis
646668
Davallia trichomanoides
mandelonitrile + D-glucose
-
-
-
?
(R)-vicianin + H2O
i.e. beta-vicianoside of (R)-mandelonitrile, strict requirement for (R)-epimers
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
(R)-vicianin + H2O
cyanogenic disaccharides preferred over monosaccharides
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
(R)-vicianin + H2O
involved in catabolism of cyanogenic glycosides
646668
Davallia trichomanoides
mandelonitrile + vicianose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
poor substrate
646668
Davallia trichomanoides
4-nitrophenol + D-glucopyranose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Davallia trichomanoides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
with p-nitrophenyl-beta-D-glucopyranoside as substrate
Davallia trichomanoides
5.5
-
with (R)-vicianin as substrate
Davallia trichomanoides
6
-
with (R)-prunazin as substrate
Davallia trichomanoides
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Davallia trichomanoides
-
4.7
4.6
Other publictions for EC 3.2.1.119
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
682350
Ahn
Vicianin hydrolase is a novel ...
Vicia sativa subsp. nigra
Plant Cell Physiol.
48
938-947
2007
-
-
1
-
-
-
-
-
-
-
2
-
-
5
-
-
1
-
-
6
3
-
6
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
6
3
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
646668
Lizotte
Catabolism of cyanogenic glyco ...
Davallia trichomanoides
Plant Physiol.
86
322-324
1988
-
-
-
-
-
-
9
3
-
-
1
1
-
2
-
1
1
-
-
2
-
-
7
-
-
1
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
9
-
3
-
-
1
1
-
-
1
1
-
2
-
-
7
-
1
-
-
-
3
-
-
1
-
-
-
-
-
-
646667
Kuroki
-
Catabolism of (R)-amygdalin an ...
Davallia trichomanoides
Z. Naturforsch. C
39c
232-239
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
8
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-