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Literature summary for 3.2.1.118 extracted from

  • Brimer, L.; Cicalini, A.R.; Federici, F.; Petruccioli, M.
    Amygdalin degradation by Mucor circinelloides and Penicillium aurantiogriseum: mechanisms of hydrolysis (1998), Arch. Microbiol., 169, 106-112.
    View publication on PubMed
Search for more literature for 3.2.1.118

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium aurantiogriseum
-
-
extracellular
-
 Mucor circinelloides
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58800
-
 PG I, SDS-PAGE Penicillium aurantiogriseum
66400
-
 PG II, SDS-PAGE Penicillium aurantiogriseum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-prunasin + H2O Penicillium aurantiogriseum
-
 mandelonitrile + D-glucose
-
 ?
(R)-prunasin + H2O Mucor circinelloides
-
 mandelonitrile + D-glucose
-
 ?
(R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Mucor circinelloides
-
-
-
Penicillium aurantiogriseum
-
-
-
Prunus serotina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium aurantiogriseum
-
 Mucor circinelloides

Reaction

Reaction Comment Organism Reaction ID
(R)-prunasin + H2O = D-glucose + mandelonitrile extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis Penicillium aurantiogriseum
a
(R)-prunasin + H2O = D-glucose + mandelonitrile extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis Mucor circinelloides
a
(R)-prunasin + H2O = D-glucose + mandelonitrile two isoforms found, PG I and PG II Mucor circinelloides
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.26
-
 purified PG I, pH 6, 25°C Penicillium aurantiogriseum
1.11
-
 purified PG II, pH 6, 25°C Penicillium aurantiogriseum
12.26
-
 purified enzyme, pH 6, 25°C Mucor circinelloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
(R)-prunasin + H2O slow reaction Penicillium aurantiogriseum D-glucose + mandelonitrile
-
 ?
(R)-prunasin + H2O slow reaction Mucor circinelloides D-glucose + mandelonitrile
-
 ?
(R)-prunasin + H2O
-
 Penicillium aurantiogriseum mandelonitrile + D-glucose
-
 ?
(R)-prunasin + H2O
-
 Mucor circinelloides mandelonitrile + D-glucose
-
 ?
(R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?