KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.65 | - |
(R)-amygdalin | AH II' | Prunus serotina | |
1.88 | - |
(R)-amygdalin | AH II | Prunus serotina | |
2.01 | - |
(R)-amygdalin | AH I | Prunus serotina | |
2.2 | - |
(R)-amygdalin | AH I' | Prunus serotina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
all isoforms, gel filtration | Prunus serotina |
60000 | - |
all isoforms, SDS-PAGE | Prunus serotina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-amygdalin + H2O | Prunus serotina | first step of cyanogenesis from amygdalin | (R)-prunasin + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus serotina | - |
Ehrh., black cherry | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Prunus serotina |
Purification (Comment) | Organism |
---|---|
four isoforms | Prunus serotina |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-amygdalin + H2O = (R)-prunasin + D-glucose | four isoforms found, AH I, AH I', AH II, AH II' | Prunus serotina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Prunus serotina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
purified AH II' | Prunus serotina |
0.48 | - |
purified AH I' and AH II | Prunus serotina |
0.58 | - |
purified AH I | Prunus serotina |
Storage Stability | Organism |
---|---|
4°C, 10 mM histidine-HCl buffer, pH 6.0 | Prunus serotina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-amygdalin + H2O | - |
Prunus serotina | (R)-prunasin + D-glucose | - |
? | |
(R)-amygdalin + H2O | first step of cyanogenesis from amygdalin | Prunus serotina | (R)-prunasin + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Prunus serotina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | all isoforms | Prunus serotina |