General Stability | Organism |
---|---|
0.5% soluble starch stabilizes | Natronococcus sp. |
2.5 M NaCl stabilizes | Natronococcus sp. |
activity is irreversibly lost at low ionic strength | Natronococcus sp. |
no loss of activity after dialysis | Natronococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-cyclodextrin | competitive inhibitor, 0.25% | Natronococcus sp. | |
beta-cyclodextrin | competitive inhibitor, 0.025% | Natronococcus sp. | |
Ca2+ | 1 mM, 33% inhibition | Natronococcus sp. | |
Hg2+ | weak, 1 mM: 15% inhibition, 10 mM: 75% inhibition | Natronococcus sp. | |
additional information | not inhibited by 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride | Natronococcus sp. | |
N-bromosuccinimide | 1 mM, 73% inhibition | Natronococcus sp. | |
Ni2+ | 1 mM, 33% inhibition | Natronococcus sp. | |
SDS | 0.1%, 80% inhibition | Natronococcus sp. | |
Urea | 1 M, 74% inhibition | Natronococcus sp. | |
ZnCl2 | 1 mM, complete inhibition | Natronococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
gamma-cyclodextrin | pH 9, 50°C, Km-value shown as glucose equivalents | Natronococcus sp. | |
19 | - |
amylose | pH 9, 50°C, DP, 17, Km-value shown as glucose equivalents | Natronococcus sp. | |
25 | - |
starch | pH 9, 50°C, soluble, Km-value shown as glucose equivalents | Natronococcus sp. | |
42 | - |
glycogen | pH 9, 50°C, from oyster, Km-value shown as glucose equivalents | Natronococcus sp. | |
49 | - |
amylopectin | pH 9, 50°C, Km-value shown as glucose equivalents | Natronococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Natronococcus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CsCl | can partially substitute for NaCl at higher concentrations | Natronococcus sp. | |
KCl | can partially substitute for NaCl at higher concentrations | Natronococcus sp. | |
NaCl | requirement, maximum activity at 2.5 M NaCl, no activity below 1 M NaCl | Natronococcus sp. | |
RbCl | can partially substitute for NaCl at higher concentrations | Natronococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
1 * 74000, SDS-PAGE | Natronococcus sp. |
76000 | - |
gel filtration | Natronococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Natronococcus sp. | - |
- |
- |
Natronococcus sp. Ah-36 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2000fold, in the presence of 2.5 M NaCl | Natronococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
pH 9, 50°C | Natronococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 glycogen + H2O | from oyster, slow hydrolysis | Natronococcus sp. | 4 maltotriose | additional small amounts of maltose and glucose, alpha-configuration | ? | |
amylopectin + H2O | - |
Natronococcus sp. | maltotriose | additional small amounts of maltose and glucose, alpha-configuration | ? | |
amylose + H2O | - |
Natronococcus sp. | maltotriose | - |
? | |
gamma-cyclodextrin + H2O | - |
Natronococcus sp. | ? | - |
? | |
maltoheptaose + H2O | - |
Natronococcus sp. | maltotriose | main product, additional small amounts of maltose and glucose, alpha-configuration | ? | |
maltohexaose + H2O | - |
Natronococcus sp. | maltotriose + ? | alpha-configuration | ? | |
maltohexaose + H2O | - |
Natronococcus sp. | maltotriose + ? | main product, additional small amounts of maltose and glucose | ? | |
maltohexaose + H2O | - |
Natronococcus sp. Ah-36 | maltotriose + ? | alpha-configuration | ? | |
maltohexaose + H2O | - |
Natronococcus sp. Ah-36 | maltotriose + ? | main product, additional small amounts of maltose and glucose | ? | |
maltooligosaccharide + H2O | ranging from maltotetraose to maltoheptaose, not: maltotriose, maltose | Natronococcus sp. | maltotriose | alpha-configuration | ? | |
maltopentaose + H2O | - |
Natronococcus sp. | maltotriose + D-maltose | alpha-configuration | ? | |
maltopentaose + H2O | - |
Natronococcus sp. | maltotriose + D-maltose | additional small amounts of glucose | ? | |
maltotetraose + H2O | - |
Natronococcus sp. | maltotriose + D-glucose | alpha-configuration, additional product: D-maltose | ? | |
additional information | not: alpha-cyclodextrin, pullulan | Natronococcus sp. | ? | - |
? | |
additional information | not: maltotriose, maltose, beta-cyclodextrin | Natronococcus sp. | ? | - |
? | |
additional information | enzyme has transferase activity using maltopentaose or maltotetraose as substrates | Natronococcus sp. | ? | - |
? | |
additional information | not: alpha-cyclodextrin, pullulan | Natronococcus sp. Ah-36 | ? | - |
? | |
additional information | not: maltotriose, maltose, beta-cyclodextrin | Natronococcus sp. Ah-36 | ? | - |
? | |
additional information | enzyme has transferase activity using maltopentaose or maltotetraose as substrates | Natronococcus sp. Ah-36 | ? | - |
? | |
short-chain amylose + H2O | DP, 17, most rapid hydrolysis | Natronococcus sp. | maltotriose | alpha-configuration | ? | |
short-chain amylose + H2O | DP, 17, most rapid hydrolysis | Natronococcus sp. | maltotriose | additional small amounts of maltose and glucose | ? | |
starch + H2O | soluble | Natronococcus sp. | maltotriose + ? | additional small amounts of maltose and glucose, alpha-configuration | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, SDS-PAGE | Natronococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Natronococcus sp. |
55 | - |
pH 9, 30 min, in the presence of 2.5 M NaCl | Natronococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
soluble starch stabilizes against thermal inactivation, Ca2+ does not enhance thermal stability | Natronococcus sp. |
50 | - |
30 min, 2.5 M NaCl, pH 4.2: 80% loss of activity, pH 5.0: 44% loss of activity, pH 6.0-8.6: stable, pH 10.5: 30% loss of activity | Natronococcus sp. |
55 | - |
pH 8.0, 30 min, 2.5 M NaCl, 50% loss of activity, no loss of activity in the presence of 0.5% soluble starch | Natronococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
in the presence of 2.5 M NaCl | Natronococcus sp. |
9 | - |
assay at | Natronococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, 80% loss of activity | Natronococcus sp. |
4.2 | - |
30 min, 50°C, 2.5 M NaCl, 80% loss of activity | Natronococcus sp. |
5 | - |
30 min, 50°C, 2.5 M NaCl, 44% loss of activity | Natronococcus sp. |
6 | 10 | 30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, stable | Natronococcus sp. |
6 | 8.6 | 30 min, 50°C, 2.5 M NaCl, stable | Natronococcus sp. |
10.5 | - |
30 min, 50°C, 2.5 M NaCl, 30% loss of activity | Natronococcus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
beta-cyclodextrin | pH 9, 50°C, 0.025% | Natronococcus sp. | |
1.63 | - |
alpha-cyclodextrin | pH 9, 50°C, 0.25% | Natronococcus sp. |