Application | Comment | Organism |
---|---|---|
nutrition | enzyme produced on the industrial scale for the production of high maltotriose-containing syrup | Microbacterium imperiale |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Microbacterium imperiale | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
x * 25500, SDS-PAGE | Microbacterium imperiale |
40000 | - |
gel filtration | Microbacterium imperiale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium imperiale | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Microbacterium imperiale |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Microbacterium imperiale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 glycogen + H2O | - |
Microbacterium imperiale | 4 maltotriose | main product | ? | |
amylopectin + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
amylose + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
beta-cyclodextrin + H2O | - |
Microbacterium imperiale | maltotriose | main product | ? | |
maltohexaose + H2O | - |
Microbacterium imperiale | maltotriose + ? | alpha-configuration | ? | |
maltooligosaccharide + H2O | hydrolysis of maltohexaose, maltopentaose, maltotetraose and maltotriose | Microbacterium imperiale | maltotriose | alpha-configuration | ? | |
maltopentaose + H2O | - |
Microbacterium imperiale | maltotriose + D-maltose | alpha-configuration | ? | |
maltotetraose + H2O | - |
Microbacterium imperiale | maltotriose + D-glucose | alpha-configuration, additional product: D-maltose | ? | |
maltotriose + H2O | slow hydrolysis | Microbacterium imperiale | maltose + D-glucose | alpha-configuration | ? | |
additional information | not: alpha-cyclodextrin, pullulan | Microbacterium imperiale | ? | - |
? | |
additional information | no hydrolysis of alpha-1,6-glucosidic linkages | Microbacterium imperiale | ? | - |
? | |
starch + H2O | soluble | Microbacterium imperiale | maltotriose + ? | initial main product, proceeded reaction results in 55-60% maltotriose, 10-20% maltose, 1-3% glucose, 20-40% higher oligosaccharides | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25500, SDS-PAGE | Microbacterium imperiale |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Microbacterium imperiale |
50 | - |
30 min | Microbacterium imperiale |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | about 50% of activity maximum at 25°C and 55°C | Microbacterium imperiale |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ protects against heat inactivation | Microbacterium imperiale |
40 | - |
unstable above | Microbacterium imperiale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Microbacterium imperiale |
7 | - |
assay at | Microbacterium imperiale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | pH 5.8: about 90% of activity maximum, pH 8.5: about 30% of activity maximum | Microbacterium imperiale |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable in the pH range | Microbacterium imperiale |