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Literature summary for 3.2.1.116 extracted from

  • Takasaki, Y.; Kitajima, M.; Tsuruta, T.; Nonoguchi, M.; Hayashi, S.; Imada, K.
    Studies on enzymic production of oligosaccharides. Part VI. Maltotriose-producing amylase from Microbacterium imperiale (1991), Agric. Biol. Chem., 55, 687-692.
No PubMed abstract available

Application

Application Comment Organism
nutrition enzyme produced on the industrial scale for the production of high maltotriose-containing syrup Microbacterium imperiale

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Microbacterium imperiale
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
x * 25500, SDS-PAGE Microbacterium imperiale
40000
-
gel filtration Microbacterium imperiale

Organism

Organism UniProt Comment Textmining
Microbacterium imperiale
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Microbacterium imperiale

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Microbacterium imperiale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 glycogen + H2O
-
Microbacterium imperiale 4 maltotriose main product ?
amylopectin + H2O
-
Microbacterium imperiale maltotriose main product ?
amylose + H2O
-
Microbacterium imperiale maltotriose main product ?
beta-cyclodextrin + H2O
-
Microbacterium imperiale maltotriose main product ?
maltohexaose + H2O
-
Microbacterium imperiale maltotriose + ? alpha-configuration ?
maltooligosaccharide + H2O hydrolysis of maltohexaose, maltopentaose, maltotetraose and maltotriose Microbacterium imperiale maltotriose alpha-configuration ?
maltopentaose + H2O
-
Microbacterium imperiale maltotriose + D-maltose alpha-configuration ?
maltotetraose + H2O
-
Microbacterium imperiale maltotriose + D-glucose alpha-configuration, additional product: D-maltose ?
maltotriose + H2O slow hydrolysis Microbacterium imperiale maltose + D-glucose alpha-configuration ?
additional information not: alpha-cyclodextrin, pullulan Microbacterium imperiale ?
-
?
additional information no hydrolysis of alpha-1,6-glucosidic linkages Microbacterium imperiale ?
-
?
starch + H2O soluble Microbacterium imperiale maltotriose + ? initial main product, proceeded reaction results in 55-60% maltotriose, 10-20% maltose, 1-3% glucose, 20-40% higher oligosaccharides ?

Subunits

Subunits Comment Organism
? x * 25500, SDS-PAGE Microbacterium imperiale

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Microbacterium imperiale
50
-
30 min Microbacterium imperiale

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 about 50% of activity maximum at 25°C and 55°C Microbacterium imperiale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Ca2+ protects against heat inactivation Microbacterium imperiale
40
-
unstable above Microbacterium imperiale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Microbacterium imperiale
7
-
assay at Microbacterium imperiale

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.5 pH 5.8: about 90% of activity maximum, pH 8.5: about 30% of activity maximum Microbacterium imperiale

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable in the pH range Microbacterium imperiale