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Literature summary for 3.2.1.115 extracted from

  • Mitsuishi , M.Y.; Kobayashi , K.M.; Matsuda , M.K.
    Dextran alpha-(1 yields 2)-debranching enzyme from Flavobacterium Sp. M-73. Properties and mode of action (1980), Carbohydr. Res., 83, 303-313.
    View publication on PubMed
Search for more literature for 3.2.1.115

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1.33 mM, 0.9% relative activity Flavobacterium sp.
Cu2+ 1.33 mM, 1.7% relative activity Flavobacterium sp.
D-glucono-1,5-lactone strong inhibitor, 34.2% relative activity Flavobacterium sp.
Fe3+ 1.33 mM, 2.6% relative activity Flavobacterium sp.
Hg2+ 1.33 mM, 1.2% relative activity Flavobacterium sp.
iodoacetic acid still 60.1% relative activity Flavobacterium sp.
Sodium dodecyl sulfate strong inhibitor, 37.9% relative activity Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 B-1298 soluble dextran mg/ml Flavobacterium sp.
0.27
-
 B-1299 soluble dextran mg/ml Flavobacterium sp.
0.91
-
 B-1397 soluble dextran mg/ml Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 gel electrophoresis Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 5.8 micro-g glucose/ml, substrate B-1298 soluble dextran Flavobacterium sp.
additional information
-
 5.8 micro-g glucose/ml, substrate B-1299 soluble dextran Flavobacterium sp.
additional information
-
 5.8 micro-g glucose/ml, substrate B-1397 soluble dextran Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O
-
 Flavobacterium sp. isomaltotetraose + 2 alpha-D-glucose
-
 ?
2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O
-
 Flavobacterium sp. isomaltotetraose + alpha-D-glucose
-
 ?
2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O
-
 Flavobacterium sp. isomaltotriose + alpha-D-glucose
-
 ?
B-1298 soluble dextran + H2O incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages Flavobacterium sp. D-glucose + ?
-
 ?
B-1299 soluble dextran + H2O incubation at 40°C for 24 h Flavobacterium sp. D-glucose + ?
-
 ?
B-1397 soluble dextran + H2O incubation at 40°C for 24 h Flavobacterium sp. D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
dextran alpha-(1-->2)-debranching enzyme
-
 Flavobacterium sp.
dextranhydrolase
-
 Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 up to 40°C on heating for 10 min Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 highest activity Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 incubation at 4°C for 24 h Flavobacterium sp.