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Literature summary for 3.2.1.114 extracted from

  • Crispin, M.; Chang, V.T.; Harvey, D.J.; Dwek, R.A.; Evans, E.J.; Stuart, D.I.; Jones, E.Y.; Lord, J.M.; Spooner, R.A.; Davis, S.J.
    A human embryonic kidney 293T cell line mutated at the Golgi alpha-mannosidase II locus (2009), J. Biol. Chem., 284, 21684-21695.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.114

Application

Application Comment Organism
biotechnology the Lec36 cell line will be useful for expressing therapeutic glycoproteins with hybrid-type glycans and as a sensitive host for detecting mutations in human MAN2A1 causing type II congenital dyserythropoietic anemia Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene MAN2A1 from mutant strain Lec36 and wild-type gene, DNA and amino acid sequence determination and analysis. Wild-type and mutant enzyme expression analysis, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the mutant gene MAN2A1 from strain Lec36 harbors a point mutation in the active site in one allele and an in-frame deletion of 12 nucleotides in the other allele, compared to parent HEK293T cells, which alters the glycosylation pattern. Expression of the wild-type but not the mutant MAN2A1 alleles in Lec36 cells restores processing of the 19A reporter glycoprotein to complex-type glycosylation, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
swainsonine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell mutant HEK293T cell line called Lec36, which displays sensitivity to ricin that lies between the parental HEK 293T cells, in which the secreted and membrane-expressed proteins are dominated by complex-type glycosylation, and 293S Lec1 cells, which produce only oligomannose-type N-linked glycans. Lec36 cells are dominated by hybrid-type glycosylation. Wild-type and mutant enzyme expression analysis, overview Homo sapiens
-

Synonyms

Synonyms Comment Organism
Golgi alpha-mannosidase II
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme is important for N-glycosylation of protein in the Golgi apparatus Homo sapiens