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Literature summary for 3.2.1.114 extracted from

  • Woo, K.K.; Miyazaki, M.; Hara, S.; Kimura, M.; Kimura, Y.
    Purification and characterization of a co(II)-sensitive alpha-mannosidase from Ginkgo biloba seeds (2004), Biosci. Biotechnol. Biochem., 68, 2547-2556.
    View publication on PubMed
Search for more literature for 3.2.1.114

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin complete inhibition at 0.5 mM Ginkgo biloba
Cu2+ complete inhibition at 1 mM Ginkgo biloba
EDTA 26% inhibition at 1 mM Ginkgo biloba
kifunensine complete inhibition at 0.5 mM Ginkgo biloba
Zn2+ 9% inhibition at 1 mM Ginkgo biloba

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 7% activation at 1 mM Ginkgo biloba
Co2+ 120% activation at 1 mM, not essential Ginkgo biloba
Fe2+ 14% activation at 1 mM Ginkgo biloba
additional information the enzyme is not affected by Mn2+ Ginkgo biloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 3 * 120000, SDS-PAGE Ginkgo biloba
340000
-
 gel filtration Ginkgo biloba

Organism

Organism UniProt Comment Textmining
Ginkgo biloba
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation, the sugar chain of the enzyme is recognized by concanavalin A, no digestion by endoglycosidase H, glycan type is deduced as GlcNAc2Man3Xyl1Fuc1GlcNAc2, bianntenary plant complex type N-glycans Ginkgo biloba

Purification (Commentary)

Purification (Comment) Organism
native enzyme 600fold from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration, and another 2 steps of ion exchange chromatography and gel filtration Ginkgo biloba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Ginkgo biloba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
 purified native enzyme Ginkgo biloba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-mannoside + H2O
-
 Ginkgo biloba 4-nitrophenol + alpha-D-mannose
-
 ?
Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
additional information substrate specificity, overview Ginkgo biloba ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 120000, SDS-PAGE Ginkgo biloba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ginkgo biloba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 8.5 low activity at neutral pH Ginkgo biloba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Ginkgo biloba