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Literature summary for 3.2.1.114 extracted from
- Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates (2003), J. Biol. Chem., 278, 48074-48083.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| wild-type and D341N mutant GMII, hanging drop vapor diffusion | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D341N | mutant with removed acid/base catalyst, 2fold lower Km and 200fold kcat values compared with the wild-type enzyme | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-2-fluoro-alpha-D-mannosyl fluoride | reversible, D341N mutant GMII | Drosophila melanogaster |  |
| 5-fluoro-beta-L-gulosyl fluoride | reversible, wild-type and D341N mutant GMII, inhibits only at low assay temperatures, acts as slow substrate at 37°C | Drosophila melanogaster | |
| swainsonine | - |
Drosophila melanogaster | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
2,4-dinitrophenyl alpha-D-mannoside | pH 5.6, 37°C, D341N mutant GMII | Drosophila melanogaster | |
| 0.2 | - |
5-fluoro-beta-L-gulosyl fluoride | pH 5.6, 37°C, wild-type GMII | Drosophila melanogaster | |
| 5.5 | - |
2,4-dinitrophenyl alpha-D-mannoside | pH 5.6, 37°C, wild-type GMII | Drosophila melanogaster | |
| 25 | - |
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride | pH 5.6, 37°C, above, wild-type GMII | Drosophila melanogaster | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | active site coordination to Zn2+ | Drosophila melanogaster | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120500 | - |
x * 120500, D341N mutant GMII | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Drosophila melanogaster | asparagine-linked glycoprotein biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q24451 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose | catalytic mechanism | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dinitrophenyl alpha-D-mannoside + H2O | - |
Drosophila melanogaster | 2,4-dinitrophenol + alpha-D-mannose | - |
? | |
| 2-deoxy-2-fluoro-alpha-D-mannosyl fluoride | wild-type and D341N mutant GMII, acts as very slow substrate of the mutant enzyme with a rate-limiting deglycosylation step | Drosophila melanogaster | ? | - |
? | |
| 5-fluoro-beta-L-gulosyl fluoride + H2O | acts as slow substrate with a rate-limiting deglycosylation step, wild-type and D341N mutant GMII | Drosophila melanogaster | ? | - |
? | |
| additional information | enzyme/active site structure and substrate binding, catalytic mechanism | Drosophila melanogaster | ? | - |
? | |
| additional information | asparagine-linked glycoprotein biosynthesis | Drosophila melanogaster | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120500, D341N mutant GMII | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GmII | - |
Drosophila melanogaster |
| More | member of glycosyl hydrolase family 38 | Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Drosophila melanogaster |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
5-fluoro-beta-L-gulosyl fluoride | pH 5.6, 37°C, wild-type GMII | Drosophila melanogaster | |
| 0.048 | - |
2,4-dinitrophenyl alpha-D-mannoside | pH 5.6, 37°C, D341N mutant GMII | Drosophila melanogaster | |
| 8.6 | - |
2,4-dinitrophenyl alpha-D-mannoside | pH 5.6, 37°C, wild-type GMII | Drosophila melanogaster | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
assay at | Drosophila melanogaster |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
5-fluoro-beta-L-gulosyl fluoride | pH 5.6, 37°C, D341N mutant GMII | Drosophila melanogaster | |
| 7.5 | - |
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride | pH 5.6, 37°C, D341N mutant GMII | Drosophila melanogaster | |
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