Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimycin | 0.4 mM, 50% inhibition | Spodoptera frugiperda | |
Cu2+ | 0.1 mM, 90% inhibition of GlcNAc(Man)5(GlcNAc)2 hydrolysis, 0.01 mM, 50% inhibition of 4-nitrophenyl alpha-D-mannoside hydrolysis; strong | Spodoptera frugiperda | |
additional information | not inhibited by EDTA | Spodoptera frugiperda | |
swainsonine | 20 nM, 50% inhibition | Spodoptera frugiperda | |
Zn2+ | 10 mM: 40% inhibition, 2.5 mM: no inhibition | Spodoptera frugiperda |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Spodoptera frugiperda | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Spodoptera frugiperda | |
additional information | not activated by Ca2+ | Spodoptera frugiperda | |
additional information | not activated by Zn2+ | Spodoptera frugiperda | |
additional information | not activated by Co2+ | Spodoptera frugiperda | |
additional information | not activated by Sr2+ | Spodoptera frugiperda | |
additional information | not activated by Mg2+, Mn2+ | Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Spodoptera frugiperda |
240000 | - |
PAGE under nonreducing conditions | Spodoptera frugiperda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Spodoptera frugiperda | asparagine-linked glycoprotein biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
baculovirus-infected IPLB-SF-21AE | - |
Purification (Comment) | Organism |
---|---|
353fold | Spodoptera frugiperda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SF-21 cell | baculovirus-infected IPLB-SF-21AE cells | Spodoptera frugiperda | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + alpha-D-mannose | - |
? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O | - |
Spodoptera frugiperda | Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose | - |
? | |
additional information | not: (Man)5(GlcNAc)2 | Spodoptera frugiperda | ? | - |
? | |
additional information | asparagine-linked glycoprotein biosynthesis | Spodoptera frugiperda | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE | Spodoptera frugiperda |
dimer | disulfide-linked homodimer | Spodoptera frugiperda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spodoptera frugiperda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
non-reduced GlcNAc(Man)5(GlcNAc)2 hydrolysis | Spodoptera frugiperda |