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Literature summary for 3.2.1.114 extracted from

  • Ren, J.; Castellino, F.J.; Bretthauer, R.K.
    Purification and properties of alpha-mannosidase II from Golgi-like membranes of baculovirus-infected Spodoptera frugiperda (IPLB-SF-21AE) cells (1997), Biochem. J., 324, 951-956.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin 0.4 mM, 50% inhibition Spodoptera frugiperda
Cu2+ 0.1 mM, 90% inhibition of GlcNAc(Man)5(GlcNAc)2 hydrolysis, 0.01 mM, 50% inhibition of 4-nitrophenyl alpha-D-mannoside hydrolysis; strong Spodoptera frugiperda
additional information not inhibited by EDTA Spodoptera frugiperda
swainsonine 20 nM, 50% inhibition Spodoptera frugiperda
Zn2+ 10 mM: 40% inhibition, 2.5 mM: no inhibition Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
Spodoptera frugiperda 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Spodoptera frugiperda
additional information not activated by Ca2+ Spodoptera frugiperda
additional information not activated by Zn2+ Spodoptera frugiperda
additional information not activated by Co2+ Spodoptera frugiperda
additional information not activated by Sr2+ Spodoptera frugiperda
additional information not activated by Mg2+, Mn2+ Spodoptera frugiperda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
2 * 120000, SDS-PAGE Spodoptera frugiperda
240000
-
PAGE under nonreducing conditions Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Spodoptera frugiperda asparagine-linked glycoprotein biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
baculovirus-infected IPLB-SF-21AE
-

Purification (Commentary)

Purification (Comment) Organism
353fold Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
SF-21 cell baculovirus-infected IPLB-SF-21AE cells Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannoside + H2O
-
Spodoptera frugiperda 4-nitrophenol + alpha-D-mannose
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O
-
Spodoptera frugiperda Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?
additional information not: (Man)5(GlcNAc)2 Spodoptera frugiperda ?
-
?
additional information asparagine-linked glycoprotein biosynthesis Spodoptera frugiperda ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE Spodoptera frugiperda
dimer disulfide-linked homodimer Spodoptera frugiperda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
non-reduced GlcNAc(Man)5(GlcNAc)2 hydrolysis Spodoptera frugiperda