Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.114 extracted from

  • Tropea, J.E.; Kaushal, G.P.; Pastuszak, I.; Mitchell, M.; Aoyagi, T.; Molyneux, R.J.; Elbein, A.D.
    Mannostatin A, a new glycoprotein-processing inhibitor (1990), Biochemistry, 29, 10062-10069.
    View publication on PubMed
Search for more literature for 3.2.1.114

Inhibitors

Inhibitors Comment Organism Structure
Mannostatin A cyclopentanol structure containing thiomethyl and amino functional groups, in vivo and in vitro Canis lupus familiaris
Mannostatin A cyclopentanol structure containing thiomethyl and amino functional groups, in vivo and in vitro; very potent inhibitor, competitive, IC50: about 10 nM with 4-nitrophenyl alpha-D-mannoside as substrate, about 90 nM with GlcNAc(Man)5GlcNAc as substrate, equally potent as swainsonine Vigna radiata
additional information not inhibited by N-acetylmannostatin A Canis lupus familiaris
additional information not inhibited by N-acetylmannostatin A Vigna radiata
swainsonine very strong Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcNAc(Man)5GlcNAc + 2 H2O Vigna radiata natural substrate, involved in the processing of N-linked oligosaccharides GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Vigna radiata
-
 mung bean
-

Source Tissue

Source Tissue Comment Organism Textmining
MDCK cell influenza virus-infected Canis lupus familiaris
-
seedling
-
 Vigna radiata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannoside + H2O
-
 Canis lupus familiaris 4-nitrophenol + alpha-D-mannose
-
 ?
4-nitrophenyl alpha-D-mannoside + H2O
-
 Vigna radiata 4-nitrophenol + alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O
-
 Canis lupus familiaris GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O
-
 Vigna radiata GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O natural substrate, involved in the processing of N-linked oligosaccharides Vigna radiata GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00001
-
 very potent inhibitor, competitive, IC50: about 10 nM with 4-nitrophenyl alpha-D-mannoside as substrate, about 90 nM with GlcNAc(Man)5GlcNAc as substrate, equally potent as swainsonine Vigna radiata Mannostatin A