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Literature summary for 3.2.1.114 extracted from
- Glycoprotein processing enzymes of plants (1989), Methods Enzymol., 179, 452-475.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activation, 0.15-0.2% | Vigna radiata |  |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes | Vigna radiata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-Dideoxy-1,4-imino-D-mannitol | - |
Vigna radiata | |
| Cu2+ | - |
Vigna radiata | |
| additional information | not inhibited by castanospermine, deoxynojirimycin; not inhibited by deoxymannojirimycin | Vigna radiata | |
| swainsonine | - |
Vigna radiata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Vigna radiata | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
x * 128000, SDS-PAGE | Vigna radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
mung bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Vigna radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vigna radiata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 weeks, stable | Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Man)3GlcNAc + H2O | poor substrate | Vigna radiata | ? | - |
? | |
| GlcNAc(Man)5GlcNAc + 2 H2O | strict substrate specificity | Vigna radiata | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | final product, via GlcNAc(Man)4GlcNAc | ? | |
| additional information | not: Glc3(Man)9GlcNAc | Vigna radiata | ? | - |
? | |
| additional information | not: (Man)9GlcNAc | Vigna radiata | ? | - |
? | |
| additional information | not: (Man)5GlcNAc | Vigna radiata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 128000, SDS-PAGE | Vigna radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.4 | - |
Vigna radiata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
below, loss of activity | Vigna radiata |
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Release 2023.1 (January 2023)
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