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Literature summary for 3.2.1.114 extracted from
- Characterization of microsomal and cytosolic alpha-1,2-mannosidases from mung bean hypocotyls (1985), Arch. Biochem. Biophys., 242, 48-57.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside | in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose | Vigna radiata var. radiata |  |
| 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside | in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose | Vigna radiata var. radiata | |
| 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside | in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose | Vigna radiata var. radiata | |
| Cu2+ | strong | Vigna radiata var. radiata | |
| EDTA | Ca2+ restores | Vigna radiata var. radiata | |
| Mannosyl-mannose disaccharide | in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose | Vigna radiata var. radiata | |
| Zn2+ | strong | Vigna radiata var. radiata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one | Vigna radiata var. radiata | 5829 | - |
| membrane | 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one | Vigna radiata var. radiata | 16020 | - |
| microsome | 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one | Vigna radiata var. radiata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Vigna radiata var. radiata | |
| Ca2+ | microsomal enzyme, activation | Vigna radiata var. radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
cytosolic enzyme, gel filtration | Vigna radiata var. radiata |
| 460000 | - |
microsomal enzyme, gel filtration, presumably in a micelle or aggregate containing detergent, phospholipid or other protein | Vigna radiata var. radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypocotyl | - |
Vigna radiata var. radiata | - |
| seedling | - |
Vigna radiata var. radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc | Vigna radiata var. radiata | (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose | - |
? | |
| additional information | no hydrolysis of alpha1,6-linkages | Vigna radiata var. radiata | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | not identical with aryl-alpha-mannosidase | Vigna radiata var. radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Vigna radiata var. radiata |
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