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Literature summary for 3.2.1.114 extracted from

  • Tulsiani, D.R.P.; Hubbard, S.C.; Robbins, P.W.; Touster, O.
    alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates (1982), J. Biol. Chem., 257, 3660-3668.
    View publication on PubMed
Search for more literature for 3.2.1.114

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 91-93% inhibition; strong Rattus norvegicus
Fe2+ 1 mM, 70-76% inhibition; strong Rattus norvegicus
Fe3+ less potent inhibitor than Fe2+ and Cu2+, 1 mM, 24-30% inhibition Rattus norvegicus
additional information not inhibited by Co2+, Zn2+; not inhibited by EDTA; not inhibited by Tris-Cl, iodoacetamide, alpha-methylmannoside; not inhibited by Tris-maleate Rattus norvegicus
p-chloromercuriphenylsulfonate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Rattus norvegicus 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation Rattus norvegicus
Zn2+ activation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus biosynthesis of complex-type N-linked oligosaccharides of glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
20fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.425
-
 4-nitrophenyl alpha-D-mannoside as substrate Rattus norvegicus

Storage Stability

Storage Stability Organism
2-4°C, 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 4 weeks, 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Man)6GlcNAc + H2O rate at 2% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
(Man)7GlcNAc + H2O rate at 3% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
(Man)8GlcNAc + H2O rate at 5% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
(Man)9GlcNAc + H2O rate at 6.4% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
4-nitrophenyl alpha-D-mannoside + H2O rate at 23.5% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus 4-nitrophenol + alpha-D-mannose
-
 ?
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Rattus norvegicus D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O preferred substrate Rattus norvegicus GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
additional information not: (Man)5GlcNAc Rattus norvegicus ?
-
 ?
additional information biosynthesis of complex-type N-linked oligosaccharides of glycoproteins Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 24 h, in 100 mM sodium acetate buffer, pH 5.8, 30-50% loss of activity Rattus norvegicus
50
-
 3 h, about 30% loss of activity, 4-nitrophenyl alpha-D-mannoside or (Man)9GlcNAc as substrate Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 4-nitrophenyl alpha-D-mannoside hydrolysis Rattus norvegicus
5.8
-
 (Man)8GlcNAc as substrate Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 1 h, at 37°C, inactivation, 1 mM ZnCl2 partially stabilizes Rattus norvegicus
6
-
 1 h, at 37°C, 30% loss of activity Rattus norvegicus
8
-
 1 h, at 37°C, stable Rattus norvegicus