General Stability | Organism |
---|---|
freezing and thawing greatly enhances the rate of cleavage of the intact enzyme | Rattus norvegicus |
ultrafiltration, even in the presence of PMSF at 0°C, results in a slow conversion of the intact enzyme to the 110 kDa degradation product, the catalytic domain, which itself is extremely stable | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
swainsonine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
4-nitrophenyl alpha-D-mannoside | intact 124 kDa Man II and chymotrypsin-cleaved 110 kDa catalytic subunit | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | integral membrane protein, hydrophilic catalytic domain is anchored to the lumenal face of Golgi membranes through an NH2-terminal hydrophobic membrane-anchoring domain | Rattus norvegicus | 139 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, chymotrypsin-cleaved catalytic subunit, SDS-PAGE | Rattus norvegicus |
124000 | - |
2 * 124000, intact enzyme, SDS-PAGE | Rattus norvegicus |
230000 | - |
chymotrypsin-cleaved 110 kDa catalytic subunit, gel filtration | Rattus norvegicus |
285000 | - |
intact enzyme, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNAc(Man)5GlcNAc + 2 H2O | Rattus norvegicus | natural substrate, involved in the processing of N-linked oligosaccharides | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
intact 124 kDa Man II and chymotrypsin-cleaved soluble 110 kDa catalytic subunit | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.75 | - |
intact 124 kDa Man II | Rattus norvegicus |
7.03 | - |
chymotrypsin-cleaved soluble 110 kDa catalytic subunit | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, intact enzyme, several weeks, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-nitrophenol + alpha-D-mannose | - |
? | |
GlcNAc(Man)5GlcNAc + 2 H2O | - |
Rattus norvegicus | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | - |
? | |
GlcNAc(Man)5GlcNAc + 2 H2O | natural substrate, involved in the processing of N-linked oligosaccharides | Rattus norvegicus | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | - |
? | |
additional information | domain structure | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 124000, intact enzyme, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 110000, chymotrypsin-cleaved catalytic subunit, SDS-PAGE | Rattus norvegicus |
dimer | disulfide-linked homodimer | Rattus norvegicus |